Jingyi Lv , Chenxu Sun , Yanxia Qiu , Yonghong Ge , Jingxin Chen
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引用次数: 0
Abstract
Carbohydrate metabolism plays an essential role in mediating quality and ripening of postharvest fruit. Previous work has demonstrated the regulatory role of methyl jasmonate (MeJA) in ripening of apple fruit. However, the role of MeJA in mediating carbohydrate metabolism of ripening apple fruit is unexplored. Our work investigated impacts of MeJA treatment (1.5 mM) on carbohydrate metabolism of apple fruit during ripening at 20 ± 0.5 °C. Our data indicated that, compared with controls, exogenous MeJA decreased starch content by increasing total amylase (AM), α-amylase (α-AM) and β-amylase (β-AM) activities during ripening. It also reduced sorbitol content by enhancing NADP-sorbitol dehydrogenase (NADP-SDH) and NAD-sorbitol dehydrogenase (NAD-SDH) activities during ripening. At the late ripening stage, exogenous MeJA promoted accumulation of sucrose, glucose and fructose. Meanwhile, it increased activities of sucrose phosphate synthase (SPS) and vacuolar acid invertase (vAINV) but decreased activities of neutral invertase (NINV), fructokinase (FRK) and hexokinase (HXK). Sucrose synthases (SUSY) activity was fluctuated after MeJA treatment during ripening. Genes involved in starch and soluble sugar metabolisms displayed diverse expression profiles after MeJA treatment during ripening. Our data indicated that exogenous MeJA can alter apple quality by mediating carbohydrate metabolism.
碳水化合物代谢对采后水果的质量和成熟起着至关重要的作用。以前的研究已经证明了茉莉酸甲酯（MeJA）在苹果果实成熟过程中的调节作用。然而，MeJA 在调解成熟苹果果实碳水化合物代谢中的作用还未得到研究。我们的研究调查了 MeJA 处理（1.5 mM）对苹果果实在 20 ± 0.5 °C 下成熟过程中碳水化合物代谢的影响。我们的数据表明，与对照组相比，外源 MeJA 在成熟过程中通过提高总淀粉酶（AM）、α-淀粉酶（α-AM）和β-淀粉酶（β-AM）的活性来降低淀粉含量。它还通过提高成熟过程中 NADP-山梨醇脱氢酶（NADP-SDH）和 NAD-山梨醇脱氢酶（NAD-SDH）的活性来降低山梨醇含量。在晚熟阶段，外源 MeJA 促进了蔗糖、葡萄糖和果糖的积累。同时，它提高了蔗糖磷酸合成酶（SPS）和液泡酸转化酶（vAINV）的活性，但降低了中性转化酶（NINV）、果糖激酶（FRK）和己糖激酶（HXK）的活性。蔗糖合成酶（SUSY）的活性在成熟期经 MeJA 处理后出现波动。参与淀粉和可溶性糖代谢的基因在成熟期经 MeJA 处理后表现出不同的表达谱。我们的数据表明，外源 MeJA 可通过介导碳水化合物代谢来改变苹果品质。
期刊介绍：
Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement.
Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB.
Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
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