Prevalence and molecular characterization of Ascaridia galli in chickens from Minoufiya Governorate, Egypt

IF 1.4 Q3 PARASITOLOGY Veterinary parasitology, regional studies and reports Pub Date : 2024-11-23 DOI:10.1016/j.vprsr.2024.101170

Mahmoud AbouLaila , Maram Mahmoud , Heba Wheeb , Makoto Igarashi , Ahmed Elkhtam , Soad Menshawy

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Abstract

Ascaridia galli causes weight loss, emaciation, anemia, decreased egg production, and sometimes, intestinal obstruction and death in birds, leading to economic losses in the poultry industry. This research aimed to record the occurrence of A. galli in free-range chickens at farmers' houses in three villages in Ashmoun City, Minoufiya Governorate, Egypt. Additionally, molecular characterization was conducted using internal transcribed spacer 1 (ITS-1) region PCR and sequence analysis. A total of 570 chickens from 570 small flocks, each ranging in size from 10 to 20 free-range chickens, were investigated for the presence of A. galli. DNA was extracted from 10 adult worms collected from Shoshai and Elmanil-Dowib villages and subjected to PCR amplification and sequence analysis. The overall occurrence of A. galli in Ashmoun City was 38 %, with Elmanil-Dowib village having the highest prevalence at 45 %. The oldest chickens, over one year old, had the highest occurrence rate at 56 %, while females showed a higher occurrence (44 %) than males (15 %). The highest occurrence was observed during winter (47 %). The mean intensity and abundance of A. galli in Ashmoun were 5.52 and 2.07, respectively. The ITS-1 sequences of A. galli from Ashmoun, Minoufiya, Egypt had up to 100 % identity and clustered within the same genetic clade as A. galli from Egypt, Poland, and Bangladesh. Haplotype and nucleotide diversity analyses revealed that the sequences from Ashmoun represent a single haplotype and show no nucleotide mutations. This study presents the first report of the occurrence and genetic characterization of A. galli in Baladi chickens in Ashmoun City, Minoufiya, Egypt, thereby enhancing the understanding of A. galli epidemiology in this district and developing effective control strategies.

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埃及米努菲亚省鸡中五倍子蛔虫的流行情况和分子特征

五倍子蛔虫（Ascaridia galli）会导致家禽体重减轻、消瘦、贫血、产蛋量下降，有时还会导致肠梗阻和死亡，给家禽业造成经济损失。本研究旨在记录埃及米努菲耶省阿什蒙市三个村庄的农户散养土鸡中出现的 A. galli。此外，还利用内部转录间隔区 1（ITS-1）PCR 和序列分析进行了分子鉴定。共调查了来自 570 个小鸡群的 570 只鸡，每群鸡的规模从 10 只到 20 只不等。从 Shoshai 村和 Elmanil-Dowib 村采集的 10 只成虫中提取了 DNA，并进行了 PCR 扩增和序列分析。阿什蒙市的 A. galli 总发生率为 38%，其中 Elmanil-Dowib 村的发生率最高，为 45%。一岁以上的老鸡发病率最高，为 56%，雌鸡发病率（44%）高于雄鸡（15%）。冬季的发生率最高（47%）。在阿什蒙，A. galli 的平均强度和丰度分别为 5.52 和 2.07。埃及米努菲耶 Ashmoun 的 A. galli 的 ITS-1 序列具有高达 100 % 的一致性，与埃及、波兰和孟加拉国的 A. galli 聚类在同一基因支系中。单倍型和核苷酸多样性分析表明，来自阿什蒙的序列代表一个单倍型，没有核苷酸突变。本研究首次报道了埃及米努菲耶阿什蒙市巴拉迪鸡中五倍子甲虫的发生和遗传特征，从而加深了对该地区五倍子甲虫流行病学的了解，并制定了有效的控制策略。

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).