Occurrence and molecular characterization of Cryptosporidium oocysts in chickens from Egypt, and a meta-analysis for Cryptosporidium infections in chickens worldwide

Abstract

Cryptosporidium is a leading cause of diarrhea in children and immunocompromised patients. Various animals and birds can also be infected with this protist, and Cryptosporidium zoonosis is common. A few reports have been published worldwide on Cryptosporidium infections in chickens. The present study surveyed Cryptosporidium infections in chickens from Dakahlia governorate, Egypt. Cryptosporidium oocysts were tested, using the Ziehl Neelsen modified acid-fast staining, in 884 chickens collected from various farms and live bird markets (LBMs) in Dakahlia governorate, Egypt. COWP (Cryptosporidium oocyst wall protein)-PCR testing coupled with isolate sequencing was conducted for 10 Cryptosporidium-positive samples. Intestinal contents of 58 (6.6 %) out of the surveyed chickens in Dakahlia, Egypt, had Cryptosporidium oocysts. The native breed (Balady) had a significantly higher prevalence than the commercial breeds. Oocysts of two different size ranges were detected; 4–5.5 × 4–5 μm and 6–7 × 5–6 μm. Two of the ten PCR-tested samples were successfully sequenced and identified as Cryptosporidium parvum. The present study conducted also the first meta-analysis describing the epidemiology, clinical disease, and species diversity of Cryptosporidium infecting chickens worldwide. The pooled Cryptosporidium prevalence in 18,579 chickens tested worldwide was 18.3 %, with insignificant geographical variations. The prevalence was two-fold higher (40.5 %) at the flock level. Chickens collected from LBMs (30.5 %) had significantly higher pooled prevalence than farmed chickens (14.4 %). Insignificant prevalence variations were detected according to the production type (broiler or layer), diagnostic method, or disease status. Three Cryptosporidium species common to infect birds were molecularly identified in 760 chickens tested worldwide: Cryptosporidium baileyi, Cryptosporidium meleagridis, and Cryptosporidium galli, with the former being the most frequently detected. Cryptosporidium parvum was detected in a few samples mostly from free-range chickens. In conclusion, potential C. parvum zoonosis from chickens is suggested in Egypt, where C. parvum is common among humans and various animals. Given that chicken has a substantial role in the global C. meleagridis zoonoses, and no bird-specific Cryptosporidium spp. were detected in the tested samples from Egypt, we do recommend a large-scale molecular survey to detect various species and genotypes infecting chickens throughout various Egyptian governorates.