Development of a sustainable extraction and storage stability of antioxidant and anticholinergic pressurized natural deep eutectic solvent extracts from Citrus reticulata leaves
Gloria Domínguez-Rodríguez , Victor M. Amador-Luna , José A. Mendiola , Fabián Parada-Alfonso , Elena Ibáñez
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引用次数: 0
Abstract
The recovery of phenolic compounds from C. reticulata leaves using pressurized liquid extraction (PLE) combined with Natural Deep Eutectic Solvents (NaDES) employing Choline chloride:Glycerol (1:2 molar ratio) as NaDES was investigated for the first time. A Box-Behnken experimental design was carried out to observe the influence of time, temperature and % water in NaDES on the extraction of compounds with antioxidant and anticholinergic (AC) capacities and only with antioxidant capacity (A). Besides, optimum extracts were compared with PLE extracts obtained under the same extraction conditions but using ethanol-/water (70:30, v/v). PLE-NaDES-AC extract obtained at 150°C during 25 min with 57.9 % water in NaDES displayed the highest in vitro antioxidant and anticholinergic capacities and the highest number of different phenolic compounds identified by HPLC-IT-MS compared with the rest of extracts. Lower extraction temperature (64°C) was necessary for obtaining extracts with only antioxidant capacity. Besides, a forced stability study showed a high protection effect of NaDES on the degradation of antioxidant phenolic compounds compared to ethanolic extracts. Results indicated that the use of PLE-NaDES is a sustainable alternative to ethanolic PLE extraction for the recovery of stable bioactive phenolic compounds.
期刊介绍：
Official Journal of the European Federation of Chemical Engineering:
Part C
FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering.
Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing.
The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those:
• Primarily concerned with food formulation
• That use experimental design techniques to obtain response surfaces but gain little insight from them
• That are empirical and ignore established mechanistic models, e.g., empirical drying curves
• That are primarily concerned about sensory evaluation and colour
• Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material,
• Containing only chemical analyses of biological materials.
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