The green shield: Trichoderma's role in sustainable agriculture against soil-borne fungal threats

Abstract

Soil-borne pathogenic fungi are a major agricultural concern, leading to significant decreases in plant yield. Chemically controlling these plants imposes environmental threats that could potentially endanger both humans and other animals. Therefore, employing biological methods in plant disease control represents a more effective alternative approach. The objective of this study was to isolate Trichoderma species from soil samples and evaluate their in vitro biocontrol efficacy against fungal pathogens viz. Fusarium oxysporum, Aspergillus niger, Rhizoctonia solani, Cladosporium cladosporioides, Alternaria alternata, Penicillium citrinum, Curvularia lunata, Fusarium metavorans, Aspergillus flavus, Penicillium chrysogenum, Nigrospora sphaerica, and Fusarium solani. The biocontrol testing efficacy of the isolates against various fungal pathogens was assessed using the dual culture technique. In this investigation various Trichoderma species were isolated from 25 soil samples and were tested against 12 soil borne fungal pathogens. The radial growth inhibition of Trichoderma harzanium and Trichoderma viride varied between (20.18% to 58.13% t), (07.01% to 67.16%) respectively. Furthermore, the culture filtrates of Trichoderma species at different concentrations (5%, 10%, 15%, and 20%) caused a significant reduction in the mycelial growth of all the tested fungal pathogens. The radial growth inhibition was more by higher concentrations in comparison to low concentrations. In the light of these observations, native Trichoderma species seems to be competent biocontrol agents and provide as a sustainable method against disease caused by soil borne plant pathogens.