Environmental enrichment attenuates maternal separation-induced excessive hoarding behavior in adult female mice

IF 3.3 3区心理学 Q1 BEHAVIORAL SCIENCES Pharmacology Biochemistry and Behavior Pub Date : 2024-11-23 DOI:10.1016/j.pbb.2024.173913

Yiming Cai , Ruofan Zhao , Yuxuan Huang , Huiping Yang , Ye Liu , Rui Yang , Xiangyu Zhang , Yiran Liu , Shu Yan , Xiaoyu Liu , Xiao Liu , Xueyong Yin , Yang Yu , Shuai Gao , Yating Li , Ye Zhao , Haishui Shi

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Abstract

Background

Previous studies have demonstrated that early life stress (ELS) impacts hoarding behavior in adult humans. This study aimed to assess the potential mitigation by environmental enrichment on hoarding behavior in rodents caused by maternal separation, thereby providing insights into therapeutic strategies for hoarding disorder.

Methods

Newborn mice were randomly divided into four groups. The control group was allowed to grow naturally. The maternal separation group underwent two weeks of maternal separation. The short-term environmental enrichment group received two weeks of environmental enrichment intervention after the two weeks of maternal separation. The long-term environmental enrichment group received five weeks of environmental enrichment intervention after the two weeks of maternal separation. Hoarding behavior was assessed during adolescence and adulthood. Hippocampal tissue from adult female mice was analyzed using LC-MS/MS-based metabolomics. Spearman correlation analysis was then performed to assess the relationship between differentially expressed metabolites and hoarding behavior.

Results

Environmental enrichment attenuates maternal separation-induced excessive hoarding behavior in adult female mice. The untargeted metabolomics of the hippocampal region in female mice showed that long-term environmental enrichment reversed multiple differential metabolites, including Substance P, which were mainly concentrated in metabolic pathways such as cancer choline metabolism, glycolipid metabolism, and linoleic acid metabolism.

Conclusions

Our findings indicate that ELS and long-term environmental enrichment have sex-dependent effects on adult hoarding behavior, potentially related to altered hippocampal metabolism. This study highlights the importance of environmental enrichment in mitigating the long-term effects of early maternal separation on hoarding behavior.

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环境富集可减轻母鼠分离引起的成年雌性小鼠过度囤积行为

背景以前的研究表明，早期生活压力（ELS）会影响成年人类的囤积行为。本研究旨在评估环境强化对啮齿动物因母体分离而产生的囤积行为的潜在缓解作用，从而为囤积症的治疗策略提供启示。对照组让其自然生长。母体分离组接受两周的母体分离。母鼠分离两周后，短期丰富环境组接受为期两周的丰富环境干预。长期丰富环境组在母婴分离两周后接受为期五周的丰富环境干预。在青春期和成年期对囤积行为进行了评估。利用基于 LC-MS/MS 的代谢组学分析了成年雌性小鼠的海马组织。结果环境富集减轻了成年雌性小鼠因母体分离引起的过度囤积行为。雌性小鼠海马区的非靶向代谢组学研究表明，长期环境富集逆转了包括物质P在内的多种差异代谢物，这些代谢物主要集中在癌胆碱代谢、糖脂代谢和亚油酸代谢等代谢途径中。这项研究强调了丰富环境在减轻早期母婴分离对囤积行为的长期影响方面的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pharmacology Biochemistry and Behavior 医学-行为科学

CiteScore

6.40

自引率

2.80%

发文量

122

审稿时长

38 days

期刊介绍： Pharmacology Biochemistry & Behavior publishes original reports in the areas of pharmacology and biochemistry in which the primary emphasis and theoretical context are behavioral. Contributions may involve clinical, preclinical, or basic research. Purely biochemical or toxicology studies will not be published. Papers describing the behavioral effects of novel drugs in models of psychiatric, neurological and cognitive disorders, and central pain must include a positive control unless the paper is on a disease where such a drug is not available yet. Papers focusing on physiological processes (e.g., peripheral pain mechanisms, body temperature regulation, seizure activity) are not accepted as we would like to retain the focus of Pharmacology Biochemistry & Behavior on behavior and its interaction with the biochemistry and neurochemistry of the central nervous system. Papers describing the effects of plant materials are generally not considered, unless the active ingredients are studied, the extraction method is well described, the doses tested are known, and clear and definite experimental evidence on the mechanism of action of the active ingredients is provided.