Quantitative pharmacodynamics functional evaluation of Chinese medicine Qizhu formula in mice with dynamic near-infrared photoacoustic imaging

IF 7.1 1区医学 Q1 ENGINEERING, BIOMEDICAL Photoacoustics Pub Date : 2024-11-14 DOI:10.1016/j.pacs.2024.100667

Qing-Juan Wu , Lan-Yu Chen , Quan-Mei Sun , Ning Wang , Dong Han , Wen-Liang Lv

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Abstract

Background & Aims

Effective anti-fibrotic drugs and new non-invasive evaluation methods for liver fibrosis (LF) are urgently needed. Our study aimed to evaluate the histological effects of the Qizhu (QZ) formula on LF and to explore a non-invasive Near-infrared photoacoustic imaging (NIR-PAI) kinetic model for liver function detection and pharmacodynamic evaluation.

Methods

C57BL/6 J mice were randomly divided into six groups (n=6). An LF model was induced by CCl₄ for 8 weeks, followed by an 8-week treatment period. Histological and serological parameters were assessed, and indocyanine green (ICG) metabolism (maximum peak time [T_max] and half-life [T_1/2]) was monitored by NIR-PAI. Spearman correlation analysis was conducted to evaluate correlations.

Results & Conclusions

Histological and serological results confirmed the anti-fibrotic effects of QZ. NIR-PAI kinetic parameters indicated that QZ shortened the T_max and T_1/2 of ICG. There were good correlations between ICG metabolism and liver histopathology. The non-invasive NIR-PAI kinetic model shows potential in liver function detection and pharmacodynamic evaluation.

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利用动态近红外光声成像技术对中药祁竹方进行小鼠药效学功能定量评价

背景& 目的肝纤维化急需有效的抗纤维化药物和新的无创评价方法。我们的研究旨在评估芪珠方对肝纤维化的组织学影响，并探索一种用于肝功能检测和药效学评价的无创近红外光声成像动力学模型。用 CCl4 诱导 LF 模型 8 周，然后进行为期 8 周的治疗。评估组织学和血清学参数，并通过近红外-PAI监测吲哚菁绿（ICG）的代谢（最大峰值时间 [Tmax] 和半衰期 [T1/2]）。结果与amp；结论组织学和血清学结果证实了 QZ 的抗纤维化作用。NIR-PAI 动力学参数表明，QZ 缩短了 ICG 的 Tmax 和 T1/2。ICG 代谢与肝组织病理学之间存在良好的相关性。无创近红外-PAI动力学模型显示了在肝功能检测和药效学评估方面的潜力。

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来源期刊

Photoacoustics Physics and Astronomy-Atomic and Molecular Physics, and Optics

CiteScore

11.40

自引率

16.50%

发文量

审稿时长

53 days

期刊介绍： The open access Photoacoustics journal (PACS) aims to publish original research and review contributions in the field of photoacoustics-optoacoustics-thermoacoustics. This field utilizes acoustical and ultrasonic phenomena excited by electromagnetic radiation for the detection, visualization, and characterization of various materials and biological tissues, including living organisms. Recent advancements in laser technologies, ultrasound detection approaches, inverse theory, and fast reconstruction algorithms have greatly supported the rapid progress in this field. The unique contrast provided by molecular absorption in photoacoustic-optoacoustic-thermoacoustic methods has allowed for addressing unmet biological and medical needs such as pre-clinical research, clinical imaging of vasculature, tissue and disease physiology, drug efficacy, surgery guidance, and therapy monitoring. Applications of this field encompass a wide range of medical imaging and sensing applications, including cancer, vascular diseases, brain neurophysiology, ophthalmology, and diabetes. Moreover, photoacoustics-optoacoustics-thermoacoustics is a multidisciplinary field, with contributions from chemistry and nanotechnology, where novel materials such as biodegradable nanoparticles, organic dyes, targeted agents, theranostic probes, and genetically expressed markers are being actively developed. These advanced materials have significantly improved the signal-to-noise ratio and tissue contrast in photoacoustic methods.