Low cost, portable, 3D printable tissue precision slicer

IF 2 Q3 ENGINEERING, ELECTRICAL & ELECTRONIC HardwareX Pub Date : 2024-11-21 DOI:10.1016/j.ohx.2024.e00611

Beatriz Martinez-Martin , Isabella Lambros , Lukas Nuesslein , Yubing Sun

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Abstract

Slicing tissue samples into thin pieces is commonly used in histology analysis and more recently for organotypic culture when tissue samples are sliced alive. Currently available devices for slicing tissue samples are either designed for fixed tissue samples at low cryogenic temperatures (e.g., Cryostats), or bulky and expensive (e.g., vibratome), preventing them from routine lab usage. Here we report a cost-effective device designed to section live tissues for subsequent culture. This device consists of components crafted from 3D-printed Nylon-12- a material suitable for autoclaving to ensure sterility. Its small footprint enhances portability, allowing for convenient placement within a biosafety cabinet for an added layer of sterility assurance. Using human pluripotent stem cells derived brain organoids as an example, we demonstrated that the device both precisely and accurately makes slices. We further validate its suitability for long-term culture by extended tissue culture following slicing. Our results indicate that brain organoid slices are viable and show improved proliferation rate compared with unsliced organoids.

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低成本、便携式 3D 可打印组织精密切片机

将组织样本切成薄片通常用于组织学分析，最近还用于活体组织样本的器官分型培养。目前可用的组织切片设备要么是为低温固定组织样本而设计的（如低温恒温器），要么是笨重昂贵的（如振荡器），因此无法在实验室中常规使用。在此，我们报告了一种经济高效的装置，该装置设计用于切片活组织以进行后续培养。该装置由 3D 打印尼龙-12（一种适合高压灭菌以确保无菌的材料）制成的部件组成。该装置占地面积小，便于携带，可方便地放置在生物安全柜中，以增加一层无菌保证。我们以人类多能干细胞衍生的脑器质性组织为例，证明了该装置能精确地制作切片。通过切片后的扩展组织培养，我们进一步验证了其长期培养的适用性。我们的研究结果表明，与未切片的器官组织相比，脑器官组织切片具有活力，并显示出更高的增殖率。

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来源期刊

HardwareX Engineering-Industrial and Manufacturing Engineering

CiteScore

4.10

自引率

18.20%

发文量

124

审稿时长

24 weeks

期刊介绍： HardwareX is an open access journal established to promote free and open source designing, building and customizing of scientific infrastructure (hardware). HardwareX aims to recognize researchers for the time and effort in developing scientific infrastructure while providing end-users with sufficient information to replicate and validate the advances presented. HardwareX is open to input from all scientific, technological and medical disciplines. Scientific infrastructure will be interpreted in the broadest sense. Including hardware modifications to existing infrastructure, sensors and tools that perform measurements and other functions outside of the traditional lab setting (such as wearables, air/water quality sensors, and low cost alternatives to existing tools), and the creation of wholly new tools for either standard or novel laboratory tasks. Authors are encouraged to submit hardware developments that address all aspects of science, not only the final measurement, for example, enhancements in sample preparation and handling, user safety, and quality control. The use of distributed digital manufacturing strategies (e.g. 3-D printing) is encouraged. All designs must be submitted under an open hardware license.