Thiol-linked hyaluronic acid-mediated encapsulation of RCR-stabilized gold nanoclusters for hyaluronidase sensing and cellular imaging

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED Carbohydrate Polymers Pub Date : 2024-11-19 DOI:10.1016/j.carbpol.2024.123038

Manivannan Madhu , Meng-Yuan Tsai , Ming-Mu Hsieh , En-Yu Lin , Wei-Bin Tseng , Chi-Yu Lu , Wei-Lung Tseng

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Abstract

Encapsulating peptide-stabilized gold nanoclusters (AuNCs) with thiolated hyaluronic acid (HA-SH) and selectively adding cysteine to the peptide sequence increased their photoluminescence. We found that peptide compositions with cysteine in the middle emitted the most. RCR-stabilized AuNCs can be purified using size-exclusion chromatography to characterize their optical characteristics, chemical composition, and possible structure. Our findings show that RCR-stabilized AuNCs have a unique chemical structure, microsecond photoluminescence lifetime, good quantum yield, and near-infrared emission peak. Due to Au-S bonding and electrostatic interactions, RCR-stabilized AuNCs were encapsulated with HA-SH to create nanocomposites. HA-SH-AuNCs had a longer emission peak, greater particle size, and better photostability than RCR-stabilized AuNCs. HAase break down HA in HA-SH-AuNCs, changing their structure and size. Thus, centrifugation makes it easier to separate HA-SH-AuNCs from HAase-digested ones. Similar to earlier sensors, HA-SH-AuNCs have great sensitivity and selectivity for HAase, with a linear range of 0.5–6.0 U/mL and a detection limit of 0.39 U/mL. They were useful for urine HAase determination, with spike recovery of 103 % to 107 %. HA-SH-AuNCs further served as a platform for targeted imaging of CD44 receptor-expressing cancer cells, demonstrating bioimaging and clinical diagnostic potential.

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硫醇连接透明质酸介导的 RCR 稳定金纳米团簇封装，用于透明质酸酶传感和细胞成像

用硫醇化透明质酸（HA-SH）包裹肽稳定金纳米团簇（AuNCs），并选择性地在肽序列中添加半胱氨酸，可增加其光致发光。我们发现，中间含有半胱氨酸的肽成分发出的荧光最多。可以使用尺寸排阻色谱法纯化 RCR 稳定的 AuNC，以确定其光学特性、化学成分和可能的结构。我们的研究结果表明，RCR 稳定的 AuNC 具有独特的化学结构、微秒级的光致发光寿命、良好的量子产率和近红外发射峰。由于 Au-S 键和静电相互作用，RCR 稳定的 AuNC 被 HA-SH 包覆，形成纳米复合材料。与 RCR 稳定的 AuNCs 相比，HA-SH-AuNCs 的发射峰更长、粒径更大、光稳定性更好。HAase 分解了 HA-SH-AuNCs 中的 HA，改变了它们的结构和大小。因此，离心更容易将 HA-SH-AuNC 与被 HA 酶消化的 HA-SH-AuNC 分离。与早期的传感器类似，HA-SH-AuNCs 对 HAase 具有很高的灵敏度和选择性，线性范围为 0.5-6.0 U/mL，检测限为 0.39 U/mL。它们可用于尿液 HAase 的检测，其峰值回收率为 103 % 至 107 %。HA-SH-AuNCs 还可用作表达 CD44 受体的癌细胞的靶向成像平台，具有生物成像和临床诊断的潜力。

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来源期刊

Carbohydrate Polymers 化学-高分子科学

CiteScore

22.40

自引率

8.00%

发文量

1286

审稿时长

47 days

期刊介绍： Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.