Investigating disturbances of the core material system in the lung-gut axis of COPD based on the transcriptomics-metabolomics-microbiomics integration strategy

IF 5.3 2区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY Arabian Journal of Chemistry Pub Date : 2024-11-17 DOI:10.1016/j.arabjc.2024.106056
Tianyang Wang , Fang Wang , Ruinan Ren , Yikun He , Qi Yu , Guoan Zhao , Jinling Zhang , Qi Liu , Ying Lyu , Weiwei Jia , Wenbao Wang , Fanchen Meng , Song Lin , Yan Lin
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Abstract

Background

Although still the significance of the lung-gut axis for COPD is increasingly highlighted, it’s urgent to ulteriorly comprehend the sophisticated disturbance of the core material system along the lung-gut axis, which is of great importance for the accurate precaution and prognosis of COPD efficiently.

Aim of the study

The purpose of this study was to analyze the information connections of the lung-gut axis, thus supporting the effective treatment of COPD.

Materials and methods

An integrated multi-omics approach was applied to explore the lung-gut axis in COPD rats. Firstly, based on transcriptomics, the ssGSEA algorithm was used to evaluate changes in pulmonary inflammatory cells. Then, the disturbances of metabolic pathways in lung and feces were revealed by the Lilikoi algorithm using LC-MS and 1H NMR metabolomics. Next, the composition and function of microbial communities in lung and feces were analyzed by 16 s rRNA sequencing. Finally, the association analysis was employed to explore the possible crosstalk between the lung and gut. Furthermore, the core material system in the lung-gut axis was described based on network topology analysis.

Result

Firstly, 1652 differential expression genes (involving in immune response-regulating signaling pathway, etc.) and 15 types of inflammatory cells (including neutrophil, etc.) were identified related to COPD. 135 pulmonary differential metabolites (involving in arachidonic acid metabolism, etc.) and 105 fecal differential metabolites (involving in alanine metabolism, etc.) were revealed by metabolomics. The f_Pasteurellaceae, etc. and g_Ruminococcus_2, etc. were identified associated with COPD in lung and gut. Finally, disturbances of the core material system, composed of macrophage, neutrophil, activated dendritic cell, myeloid derived suppressor cell, arachidonic acid metabolism, alpha linolenic acid & linoleic acid metabolism, g_Psychrobacter in lung and bile secretion, p_Proteobacteria in gut, were obtained to analyze the possible information flow of the lung-gut axis.

Conclusion

The core material system for the lung-gut axis have been revealed, which might contribute to the illustration of the pathogenesis of COPD. In the future, more researches are required on the impact of the core material system in the lung-gut axis on the onset and recovery process of COPD, suggesting more precise identifying effective treatments for the disease.

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基于转录组学-代谢组学-微生物组学整合策略的慢性阻塞性肺疾病肺-肠轴核心物质系统紊乱研究
背景尽管肺-肠轴对慢性阻塞性肺疾病的意义仍日益凸显,但亟需进一步了解肺-肠轴核心物质系统的复杂紊乱,这对准确预防和有效预后慢性阻塞性肺疾病具有重要意义。研究目的本研究旨在分析肺-肠轴的信息联系,从而支持慢性阻塞性肺疾病的有效治疗。首先,基于转录组学,使用ssGSEA算法评估肺部炎症细胞的变化。然后,利用 LC-MS 和 1H NMR 代谢组学,通过 Lilikoi 算法揭示了肺和粪便中代谢途径的紊乱。接着,通过 16 s rRNA 测序分析了肺和粪便中微生物群落的组成和功能。最后,利用关联分析探讨了肺和肠道之间可能存在的串联。结果首先,确定了与慢性阻塞性肺病相关的 1652 个差异表达基因(涉及免疫反应调节信号通路等)和 15 种炎症细胞(包括中性粒细胞等)。代谢组学发现了 135 个肺部差异代谢物(涉及花生四烯酸代谢等)和 105 个粪便差异代谢物(涉及丙氨酸代谢等)。在肺部和肠道中发现了与慢性阻塞性肺病相关的 f_Pasteurellaceae 等和 g_Ruminococcus_2 等。最后,通过对由巨噬细胞、中性粒细胞、活化树突状细胞、髓样衍生抑制细胞、花生四烯酸代谢、α-亚麻酸代谢、亚油酸代谢、肺部 g_Psychrobacter 和胆汁分泌、肠道 p_Proteobacteria 等组成的核心物质系统的干扰,分析了肺-肠轴可能的信息流。今后,还需要进一步研究肺-肠轴核心物质系统对慢性阻塞性肺疾病发病和康复过程的影响,从而提出更精确、更有效的治疗方法。
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来源期刊
Arabian Journal of Chemistry
Arabian Journal of Chemistry CHEMISTRY, MULTIDISCIPLINARY-
CiteScore
10.80
自引率
3.30%
发文量
763
审稿时长
63 days
期刊介绍: The Arabian Journal of Chemistry is an English language, peer-reviewed scholarly publication in the area of chemistry. The Arabian Journal of Chemistry publishes original papers, reviews and short reports on, but not limited to: inorganic, physical, organic, analytical and biochemistry. The Arabian Journal of Chemistry is issued by the Arab Union of Chemists and is published by King Saud University together with the Saudi Chemical Society in collaboration with Elsevier and is edited by an international group of eminent researchers.
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