In Vivo Test to Evaluate the Cytotoxicity and Genotoxicity of Natural Zeolite (NZ) Functionalized with Silver Nanoparticles (NZ-AgNPs) on Erythrocytes of Yellowtail Tetra Fish Astyanax lacustris.

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Biological Trace Element Research Pub Date : 2024-11-25 DOI:10.1007/s12011-024-04451-7

Josiane Rodrigues Rocha da Silva, Camila Oliveira de Andrade, Anna Carla Ribeiro, Paula Derksen Macruz, Elizabeth da Costa Neves FernandesAlmeida Duarte, Rosângela Bergamasco, Ana Luiza de Brito Portela-Castro, Carlos Alexandre Fernandes

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Abstract

Silver nanoparticles (AgNPs) have unique properties such as chemical stability, malleability, flexibility, high electrical and thermal conductivity, and catalytic activity, making them suitable for various applications. However, they also pose potential toxicity and environmental contamination risks. Natural zeolites (NZs) are considered excellent inorganic support for reducing the toxicity of AgNPs. The scope of this study was to carry out a comparative assessment of the cytotoxic and genotoxic effects of NZ, NZ functionalized with silver nanoparticles (NZ-AgNPs), and commercial AgNPs on fish Astyanax lacustris. Micronucleus (MN) test, cellular morphological change (CMC) test, and the comet assay were used to assess the effects of the nanoparticles. A. lacustris specimens were exposed to a concentrations of 0.1 mg l^-1 in the NZs, NZ-AgNPs, and commercial AgNPs for 96 h during the experiment. The results showed no significant difference between the negative control and NZ. Moreover, NZ-AgNPs exhibited reduced toxicities compared to commercial AgNPs, which caused higher levels of alterations and cellular damage. The study concluded that NZs effectively reduced cytotoxicity/genotoxicity in A. lacustris specimens when used to support AgNPs.

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评估银纳米颗粒（NZ-AgNPs）功能化天然沸石（NZ）对黄尾四大家鱼（Astyanax lacustris）红细胞的细胞毒性和遗传毒性的体内试验。

银纳米粒子（AgNPs）具有独特的性能，如化学稳定性、延展性、柔韧性、高导电性和导热性以及催化活性，因此适合各种应用。然而，它们也具有潜在的毒性和环境污染风险。天然沸石（NZs）被认为是降低 AgNPs 毒性的绝佳无机支持物。本研究的目的是比较评估天然沸石、银纳米粒子功能化天然沸石（NZ-AgNPs）和商用 AgNPs 对鱼类 Astyanax lacustris 的细胞毒性和遗传毒性影响。微核试验（MN）、细胞形态变化试验（CMC）和彗星试验被用来评估纳米颗粒的影响。在实验过程中，将 A. lacustris 试样暴露于浓度为 0.1 mg l-1 的 NZs、NZ-AgNPs 和商用 AgNPs 中 96 小时。结果表明，阴性对照与 NZ 之间无明显差异。此外，与商用 AgNPs 相比，NZ-AgNPs 的毒性更低，而商用 AgNPs 则会引起更高水平的变化和细胞损伤。研究得出结论，NZs 用于支持 AgNPs 时，可有效降低 A. lacustris 标本的细胞毒性/遗传毒性。

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来源期刊

Biological Trace Element Research 生物-内分泌学与代谢

CiteScore

8.70

自引率

10.30%

发文量

459

审稿时长

2 months

期刊介绍： Biological Trace Element Research provides a much-needed central forum for the emergent, interdisciplinary field of research on the biological, environmental, and biomedical roles of trace elements. Rather than confine itself to biochemistry, the journal emphasizes the integrative aspects of trace metal research in all appropriate fields, publishing human and animal nutritional studies devoted to the fundamental chemistry and biochemistry at issue as well as to the elucidation of the relevant aspects of preventive medicine, epidemiology, clinical chemistry, agriculture, endocrinology, animal science, pharmacology, microbiology, toxicology, virology, marine biology, sensory physiology, developmental biology, and related fields.