Characterization of tailocins of Pragia fontium 24613 and the tailocin loci within the family Budviciaceae.

IF 2.5 4区生物学 Q3 MICROBIOLOGY Research in microbiology Pub Date : 2024-11-23 DOI:10.1016/j.resmic.2024.104261

Kateřina Snopková, Eva Chaloupková, Matěj Hrala, David Šmajs

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Abstract

Tailocins are nano-scale phage tail-like protein complexes that can mediate antagonistic interactions between closely related bacterial species. While the capacity to produce R-type tailocin was found widely across Gammaproteobacteria, the production of F-type tailocins seems comparatively rare. In this study, we examined the freshwater isolate, Pragia fontium 24613, which can produce both R- and F-type tailocins. We investigated their inhibition spectrum, focusing on clinically relevant enterobacteria, and identified the associated tailocin gene cluster. Transmission electron microscopy confirmed that inactivation of the tape measure protein within the tailocin cluster disrupted R-tailocin production. Comparative analysis of Budviciaceae gene clusters showed high conservation of R-type tailocin genes, whereas F-type tailocin genes were found in only a few species, with little conservation. Our findings indicate a high prevalence of bacteriocin production among underexplored Enterobacteriales species. Detected tailocins showed potential as antimicrobials targeting clinically significant pathogens.

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Pragia fontium 24613 的尾丝素特征及芽苣苔科（Budviciaceae）内的尾丝素基因座。

尾蛋白是纳米级的噬菌体尾部蛋白复合物，可介导密切相关的细菌物种之间的拮抗相互作用。虽然 R 型尾蛋白的产生能力广泛存在于伽马蛋白杆菌中，但 F 型尾蛋白的产生似乎相对罕见。在本研究中，我们研究了淡水分离物 Pragia fontium 24613，它既能产生 R 型尾丝菌素，也能产生 F 型尾丝菌素。我们研究了它们对临床相关肠杆菌的抑制谱，并确定了相关的尾丝菌素基因簇。透射电子显微镜证实，尾球菌素基因簇中的胶带测量蛋白失活会破坏 R 型尾球菌素的产生。对芽胞杆菌科基因簇的比较分析表明，R型尾丝菌素基因的保存率很高，而F型尾丝菌素基因仅在少数物种中发现，保存率很低。我们的研究结果表明，在未被充分开发的肠杆菌属菌株中，细菌素的产生率很高。检测到的尾丝菌素具有作为针对临床上重要病原体的抗菌素的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in microbiology 生物-微生物学

CiteScore

4.10

自引率

3.80%

发文量

审稿时长

16 days

期刊介绍： Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.