Caries affected Dentin biomodification using Bromelain, Riboflavin photosensitizer via UVA, and Chitosan Nanoparticles on shear bond strength and microleakage scores: A SEM evaluation

Abstract

Aims

Assessment of the impact of different dentin bio-modifiers i.e., Bromelain, Riboflavin photosensitizer (RFP/Ultraviolet-A), and Chitosan nanoparticles (CHNPs) on the shear bond strength (SBS) and microleakage of composite bonded to acid etched carious affected dentin (CAD).

Materials and methods

Sixty-four human molars in which carious lesions extend till the middle third of the dentin were included. The infected dentin was removed and CAD was exposed. All specimens were categorized into four groups based on the application of dentin bio-modifier (n = 16) Group 1 (No dentin modifier), Group 2 (Bromelain), Group 3 (RFP/UVA), and Group 4 (CHNPs). Dental adhesive application and composite buildup were performed. A dye penetration test was used to assess marginal leakage. Resin CAD interface assessment was performed using SEM. For SBS and failure pattern analysis, a universal testing machine and stereomicroscope were used respectively. One-way ANOVA followed by Bonferroni's posthoc test was employed to analyze the differences among various groups (p ≤ 0.05)

Results

The lowest microleakage (13.27 ± 0.10) and highest SBS (11.64 ± 0.16 MPa) were observed in Group 2 (Bromelain). However, the maximum values of marginal leakage (37.21 ± 0.21) and minimum SBS (7.34 ± 0.07 MPa) were detected in Group 1 samples. The length of resin tags in the Group 2 specimens (Bromelain) was found to be the highest (102.11 ± 5.12 μm)^. However, Group 1 (No modifier) exhibited the lowest resin tag length (50.45 ± 2.37 μm).

Conclusion

Caries-affected dentin modified with Bromelain resulted in satisfactory SBS with minimal microleakage scores. Chitosan nanoparticles and RFP/Ultraviolet-A also presented better outcomes in terms of microleakage and SBS than the control.