High frequency of point mutations in the nitroreductase 4 and 6 genes of Trichomonas vaginalis associated with metronidazole resistance.

IF 1.5 4区医学 Q3 PARASITOLOGY Folia Parasitologica Pub Date : 2024-11-07 DOI:10.14411/fp.2024.021

Isabel Iturrieta-Gonzalez, Pamela Olivares-Ferretti, Alejandro Hidalgo, Fabiola Zambrano, Ximena Ossa, Flery Fonseca-Salamanca, Angelica Melo

引用次数: 0

Abstract

Trichomoniasis, a globally distributed sexually transmitted infection, is caused by the urogenital parasite Trichomonas vaginalis Donné, 1836 affecting both women and men. The treatment of choice is metronidazole (MTZ). In the present study, 15 samples of vaginal discharge and urine were analysed by sequencing nitroreductase genes (ntr4 and ntr6). An in silico model was structured to illustrate the location of point mutations (PM) in the protein. The ntr4 gene presented four PMs: G76C (10/10), C213G (9/10), C318A (5/10) and G424A (1/10), while the ntr6 gene had eight PMs; G593A (13/13) the most frequent, G72T and G627C, both in 8/13. The PM C213G and A438T generated a stop codon causing a truncated nitroreductase 4 and 6 protein. Docking analysis demonstrated that some models had a decrease in binding affinity to MTZ (p < 0.0001). A high frequency of mutations was observed in the samples analysed that could be associated with resistance to MTZ in Chile.

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与甲硝唑耐药性相关的阴道毛滴虫硝基还原酶 4 和 6 基因的高频点突变。

滴虫病是一种全球分布的性传播感染，由 1836 年发现的阴道毛滴虫引起，男女均可感染。治疗首选甲硝唑（MTZ）。本研究对 15 份阴道分泌物和尿液样本进行了硝基还原酶基因（ntr4 和 ntr6）测序分析。研究人员构建了一个硅学模型，以说明蛋白质中点突变（PM）的位置。ntr4 基因出现了四个点突变：而 ntr6 基因有 8 个点突变：G593A（13/13）最常见，G72T 和 G627C 均为 8/13。PM C213G 和 A438T 产生了一个终止密码子，导致硝基还原酶 4 和 6 蛋白截短。对接分析表明，一些模型与 MTZ 的结合亲和力下降（p < 0.0001）。在分析的样本中观察到了高频率的突变，这些突变可能与智利对 MTZ 的耐药性有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Folia Parasitologica 医学-寄生虫学

CiteScore

2.70

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： FOLIA PARASITOLOGICA, issued in online versions, is an international journal that covers the whole field of general, systematic, ecological and experimental parasitology. It publishes original research papers, research notes and review articles. Contributions from all branches of animal parasitology, such as morphology, taxonomy, biology, biochemistry, physiology, immunology, molecular biology and evolution of parasites, and host-parasite relationships, are eligible. Novelty and importance in the international (not local or regional) context are required. New geographical records of parasites, records of new hosts, regional parasite and/or host surveys (if they constitute the principal substance of manuscript), local/regional prevalence surveys of diseases, local/regional studies on epidemiology of well known diseases and of parasite impact on human/animal health, case reports, routine clinical studies and testing of established diagnostic or treatment procedures, will not be considered. One species description will also not be considered unless they include more general information, such as new diagnostic characters, host-parasite associations, phylogenetic implications, etc. Manuscripts found suitable on submission will be reviewed by at least two reviewers.