In-situ oxidative polymerization of dopamine triggered by CuO2 in acidic condition and application in “turn-off” sensing

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2024-11-26 DOI:10.1007/s00604-024-06791-6

Yang Huang, Shangming Wu, Siyi Yang, Miao He, Wenhao Ma, Huanbao Fa, Yongzhong Wang, Changjun Hou, Mei Yang

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Abstract

Dopamine (DA) is a key neurotransmitter whose concentration affects various neurological disorders. Unlike previous methods that synthesize non-fluorescent polydopamine (NFL-PDA) under alkaline conditions, this study introduces a novel “turn-off” sensing method for DA using NFL-PDA synthesized through a unique reaction pathway. In our approach, CuO₂ nanodots, created via a simple reduction method, catalyze the formation of hydroxyl radicals (•OH) in acidic conditions, triggering the oxidative polymerization of DA into NFL-PDA. The reaction between DA and CuO₂ nanodots in acidic solution was examined to understand the process. UiO-66-NH₂ was then used to test NFL-PDA’s fluorescence quenching ability and to further investigate the DA determination mechanism. Results showed that fluorescence quenching was due to enhanced non-radiative energy transfer and Förster resonance energy transfer (FRET) between NFL-PDA and UiO-66-NH₂. This led to the development of a simple “turn-off” fluorometric DA determination method with a linear range of 0.1–200 μmol/L and a limit of detection (LOD) of 0.0575 μmol/L. Although this method does not outperform existing NFL-PDA synthesis methods under alkaline conditions, it provides a new synthesis approach and application for sensing DA, contributing to the basic theory of chemical sensing. Additionally, DA determination in human serum samples was successfully achieved, with results consistent with high-performance liquid chromatography (HPLC).

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酸性条件下 CuO2 触发的多巴胺原位氧化聚合及其在 "关闭 "传感中的应用

多巴胺（DA）是一种关键的神经递质，其浓度会影响各种神经系统疾病。与以往在碱性条件下合成非荧光多巴胺（NFL-PDA）的方法不同，本研究利用通过独特反应途径合成的 NFL-PDA 引入了一种新型 DA "关闭 "传感方法。在我们的方法中，通过简单还原法生成的 CuO2 纳米点在酸性条件下催化羟基自由基（-OH）的形成，从而引发 DA 氧化聚合成 NFL-PDA。为了了解这一过程，我们研究了酸性溶液中 DA 与 CuO2 纳米点之间的反应。然后用 UiO-66-NH2 测试 NFL-PDA 的荧光淬灭能力，并进一步研究 DA 的测定机制。结果表明，荧光淬灭是由于 NFL-PDA 与 UiO-66-NH2 之间的非辐射能量转移和佛斯特共振能量转移（FRET）增强所致。由此开发出一种简单的 "关断 "荧光测定 DA 的方法，其线性范围为 0.1-200 μmol/L，检出限（LOD）为 0.0575 μmol/L。虽然该方法在碱性条件下的性能不优于现有的NFL-PDA合成方法，但它为DA的传感提供了一种新的合成方法和应用，为化学传感的基础理论做出了贡献。此外，还成功实现了人血清样品中DA的测定，结果与高效液相色谱法（HPLC）一致。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.