HLA class I and class II alleles and haplotypes of Algerian population from Algiers and neighbouring area

IF 1.4 4区医学 Q4 HEMATOLOGY Transfusion Clinique et Biologique Pub Date : 2025-02-01 DOI:10.1016/j.tracli.2024.11.007

Malika Bouali-Benhalima , Meriem Abbadi , Sarah Hamada , Celine Mandier , Souhila Amoura , Valérie Dubois , Sofiane Samir Salah

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Abstract

In this study, we aimed to investigate the current genetic diversity and provide additional insights into the origins of the Algerian population by analyzing the frequencies of HLA -A,-B,-DRB1,-DQB1 alleles and associated haplotypes.

We analyzed 1,082 unrelated healthy Algerian individuals, who were potential kidney donors, recruited and assessed in the Immunology Department of CHU Mustapha in Algiers over a 10-year period (2009–2019). HLA genotyping was performed by Polymerase Chain Reaction Sequence Specific Primers (PCR-SSP). The allelic typing estimate and haplotype frequencies were calculated using the R studio® software and Haplotype frequency estimation from the website https://hla-net.eu/.

We identified a total of 20 HLA-A alleles, 31 HLA-B alleles, 13 HLA-DRB1 alleles and5 HLA-DQB1 alleles.

Regarding the allele frequency, the haplotype frequency and the distribution with other population database, we were able to provide a description of Algerian genetic background and draw some anthropogenetic conclusions.

This study of HLA polymorphism in the Algerian population shows a strong genetic similarity with the populations of southern Europe and North Africa (Morocco, Tunisia and Libya) with which it has shared a common history since antiquity.

In addition to its contribution to anthropogenetics, our study, serve as a reference database for studies exploring associations between the HLA system and certain pathologies.

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阿尔及尔及邻近地区阿尔及利亚人口的 HLA I 类和 II 类等位基因及单倍型。

在这项研究中，我们旨在通过分析 HLA -A、-B、-DRB1、-DQB1 等位基因和相关单倍型的频率，调查阿尔及利亚人口目前的遗传多样性，并为了解其起源提供更多信息。我们分析了 10 年间（2009 年至 2019 年）在阿尔及尔穆斯塔法医院免疫学部招募和评估的 1082 名可能捐献肾脏的非亲属关系健康阿尔及利亚人。HLA 基因分型通过聚合酶链式反应序列特异性引物（PCR-SSP）进行。使用 R studio® 软件计算等位基因分型估计值和单倍型频率，并通过网站 https://hla-net.eu/ 估算单倍型频率。我们共鉴定出 20 个 HLA-A 等位基因、31 个 HLA-B 等位基因、13 个 HLA-DRB1 等位基因和 5 个 HLA-DQB1 等位基因。关于等位基因频率、单倍型频率以及与其他人口数据库的分布情况，我们能够对阿尔及利亚的遗传背景进行描述，并得出一些人类遗传学结论。这项关于阿尔及利亚人口 HLA 多态性的研究表明，阿尔及利亚人口与南欧和北非（摩洛哥、突尼斯和利比亚）的人口在遗传方面有很强的相似性，阿尔及利亚自古以来就与这些国家有着共同的历史。除了对人类遗传学的贡献外，我们的研究还可作为研究 HLA 系统与某些病症之间关系的参考数据库。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Transfusion Clinique et Biologique 医学-免疫学

CiteScore

2.50

自引率

11.80%

发文量

234

审稿时长

36 days

期刊介绍： Transfusion Clinique et Biologique, the official journal of the French Society of Blood Transfusion (SFTS): - an aid to training, at a European level - the only French journal indexed in the hematology and immunology sections of Current Contents Transfusion Clinique et Biologique spans fundamental research and everyday practice, with articles coming from both sides. Articles, reviews, case reports, letters to the editor and editorials are published in 4 editions a year, in French or in English, covering all scientific and medical aspects of transfusion: immunology, hematology, infectious diseases, genetics, molecular biology, etc. And finally, a convivial cross-disciplinary section on training and information offers practical updates. Readership: "Transfusers" are many and various: anesthetists, biologists, hematologists, and blood-bank, ICU and mobile emergency specialists...

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