Gonyaulax montresoriae sp. nov. (Dinophyceae) from the Adriatic Sea produces predominantly yessotoxin

Abstract

Yessotoxin is one of the shellfish toxins leading to mussel farm closures in the Adriatic Sea of Italy. Two putative Gonyaulax spinifera strains GSA0501 and GSA0602 are known as yessotoxins producers, but their identities have remained elusive since 2005. To address this gap, we established five Gonyaulax strains by incubating sediments from the Adriatic Sea and subsequently isolating single cells. Both cyst and theca morphology were examined using light and scanning electron microscopy. In addition, LSU and/or SSU rRNA gene sequences were obtained for all strains. Two strains produce cysts resembling Spiniferites mirabilis and one strain was related to S. scabratus. The other two strains are described as Gonyaulax montresoriae sp. nov., characterized by a high cingular displacement and overhang, along with two unequal antapical spines. Cysts of G. montresoriae are pear-shaped, showing a smooth surface and exclusively gonal processes with perforations at the base, the latter similar to S. lazus. LSU rRNA gene sequence comparison suggests that the G. spinifera strain GSA0501 isolated from the Adriatic Sea in 2005 should also be identified as G. montresoriae. Maximum likelihood and Bayesian inference analyses based on LSU and SSU rRNA gene sequences reveal that G. montresoriae is monophyletic, and close to several toxic strains of presumable Gonyaulax spinifera from the Adriatic Sea and New Zealand, whose taxonomic positions are uncertain. One strain of G. montresoriae was examined for yessotoxin production using LC-MS/MS, and found to produce predominantly yessotoxin at a concentration of 3.0 pg cell^-1. Our results highlight the rich diversity and risks associated with Gonyaulax species in the Mediterranean Sea.