Modified trident-shaped electrode design for particle lateral position detection in microfluidic impedance flow cytometry

IF 4.1 3区工程技术 Q2 ENGINEERING, ELECTRICAL & ELECTRONIC Sensors and Actuators A-physical Pub Date : 2024-11-26 DOI:10.1016/j.sna.2024.116062

Yunhao Peng, Bruck K. Gale, Marc D. Porter, Ling Zang, Himanshu J. Sant

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Abstract

Modified trident-shaped planar electrodes were developed for the detection of particles and their lateral positions inside a microfluidic flow channel. The electrodes generated time-of-flight measurements of individual moving particles in the form of a double peak, where each peak varies in amplitude and duration depending on particle trajectories. The amplitude ratio of the two peaks indicates the lateral positions of particles when crossing the electrode pairs. In particular, the ratio is 1 for particles near the channel centerline, >1 for particles displaced laterally to one-half of the channel, and < 1 for particles to the opposite half. This was confirmed by numerical simulations and detection of microspheres (6 – 10 µm ø) using proposed electrodes embedded in a polydimethyl siloxane (PDMS) microfluidic device. The device could count individual microspheres at a rate of at least 1151 per minute while identifying microspheres of the same material but differing in size based on peak amplitudes.

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用于微流体阻抗流式细胞仪中粒子横向位置检测的改进型三叉形电极设计

为检测微流体流动通道内的颗粒及其横向位置，开发了改进型三叉平面电极。电极以双峰形式对单个移动粒子进行飞行时间测量，每个峰的振幅和持续时间随粒子轨迹的不同而变化。两个峰值的振幅比表示粒子穿过电极对时的横向位置。具体来说，靠近通道中心线的粒子的振幅比为 1，横向移至通道二分之一处的粒子的振幅比为 1，移至通道另一半处的粒子的振幅比为 1。数值模拟和使用嵌入聚二甲基硅氧烷（PDMS）微流体装置中的电极检测微球（6 - 10 µm ø）证实了这一点。该装置能以每分钟至少 1151 个的速度对单个微球进行计数，同时还能根据峰值振幅识别相同材料但大小不同的微球。

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来源期刊

Sensors and Actuators A-physical 工程技术-工程：电子与电气

CiteScore

8.10

自引率

6.50%

发文量

630

审稿时长

49 days

期刊介绍： Sensors and Actuators A: Physical brings together multidisciplinary interests in one journal entirely devoted to disseminating information on all aspects of research and development of solid-state devices for transducing physical signals. Sensors and Actuators A: Physical regularly publishes original papers, letters to the Editors and from time to time invited review articles within the following device areas: • Fundamentals and Physics, such as: classification of effects, physical effects, measurement theory, modelling of sensors, measurement standards, measurement errors, units and constants, time and frequency measurement. Modeling papers should bring new modeling techniques to the field and be supported by experimental results. • Materials and their Processing, such as: piezoelectric materials, polymers, metal oxides, III-V and II-VI semiconductors, thick and thin films, optical glass fibres, amorphous, polycrystalline and monocrystalline silicon. • Optoelectronic sensors, such as: photovoltaic diodes, photoconductors, photodiodes, phototransistors, positron-sensitive photodetectors, optoisolators, photodiode arrays, charge-coupled devices, light-emitting diodes, injection lasers and liquid-crystal displays. • Mechanical sensors, such as: metallic, thin-film and semiconductor strain gauges, diffused silicon pressure sensors, silicon accelerometers, solid-state displacement transducers, piezo junction devices, piezoelectric field-effect transducers (PiFETs), tunnel-diode strain sensors, surface acoustic wave devices, silicon micromechanical switches, solid-state flow meters and electronic flow controllers. Etc...