LncRNA81246 regulates resistance against tea leaf spot by interrupting the miR164d-mediated degradation of NAC1.

IF 6.2 1区生物学 Q1 PLANT SCIENCES The Plant Journal Pub Date : 2024-11-26 DOI:10.1111/tpj.17173

Di Guo, Dongxue Li, Fenghua Liu, Yue Ma, Jing-Jiang Zhou, Sujitraj Sheth, Baoan Song, Zhuo Chen

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Abstract

Non-coding RNAs play crucial roles in plant responses to viral stresses. However, their molecular mechanisms in tea leaf spot responses remain unclear. In this study, using Camellia sinensis, we identified lncRNA81246 as a long non-coding RNA that localizes to both the nucleus and cytoplasm. It functions as a competitive endogenous RNA, thereby disrupting CsNAC1 (encoding NAC domain-containing protein 1) degradation mediated by miR164d. Silencing lncRNA81246 increased the resistance of tea plants to presistanceathogens, whereas transient lncRNA81246-overexpression plants showed decreased resistance to pathogens. Co-expression assays in Nicotiana benthamiana revealed that lncRNA81246 affects the miR164d-CsNAC1 regulatory module. Transient miR164d-overexpression and silencing assays demonstrated its positive regulation of tea plant resistance. Specifically, silencing its target, CsNAC1,enhanced disease resistance, whereas transient overexpression reduced plant resistance. Yeast one-hybrid, dual-luciferase, and RT-qPCR assay results suggested that CsNAC1 alters the expression of CsEXLB1, whereas AsODN and tobacco transient overexpression assays showed that CsEXLB1 negatively regulated tea plant resistance. Thus, our research demonstrated that lncRNA81246 acts as a mediator to interfere with the miR164d-CsNAC1 regulatory module involved in the disease resistance of tea plants.

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LncRNA81246 通过干扰 miR164d 介导的 NAC1 降解来调节茶叶对叶斑病的抗性。

非编码 RNA 在植物对病毒胁迫的反应中起着至关重要的作用。然而，它们在茶叶斑点病反应中的分子机制仍不清楚。在这项研究中，我们利用山茶花鉴定出了lncRNA81246，它是一种同时定位于细胞核和细胞质的长非编码RNA。它作为一种竞争性的内源性 RNA，从而干扰了 miR164d 介导的 CsNAC1（编码 NAC 结构域的蛋白 1）降解。沉默lncRNA81246可提高茶树对病原菌的抗性，而瞬时表达lncRNA81246的植株则会降低对病原菌的抗性。在Nicotiana benthamiana中的共表达实验表明，lncRNA81246影响miR164d-CsNAC1调控模块。瞬时miR164d-外表达和沉默实验证明了它对茶树抗性的正向调控作用。具体来说，沉默其靶标 CsNAC1 可增强抗病性，而瞬时过表达则会降低植物的抗病性。酵母单杂交、双荧光素酶和 RT-qPCR 检测结果表明，CsNAC1 改变了 CsEXLB1 的表达，而 AsODN 和烟草瞬时过表达检测表明，CsEXLB1 负向调控茶树的抗性。因此，我们的研究表明，lncRNA81246作为介质干扰了参与茶树抗病的miR164d-CsNAC1调控模块。

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来源期刊

The Plant Journal 生物-植物科学

CiteScore

13.10

自引率

4.20%

发文量

415

审稿时长

2.3 months

期刊介绍： Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.