A Stinkbug Salivary Protein Is Indispensable for Insect Feeding and Activates Plant Immunity.

IF 6 1区生物学 Q1 PLANT SCIENCES Plant, Cell & Environment Pub Date : 2024-11-26 DOI:10.1111/pce.15308

Guoyi Wang, Biao Hu, Xiang Yao, Zhongyan Wei, Jianping Chen, Zongtao Sun

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Abstract

Salivary proteins secreted by phytophagous insects play pivotal roles in plant-insect interactions. A salivary protein RpSP27, from the stinkbug Riptortus pedestris, a devastating pest on soybean, was selected for studying due to its ability to induce cell death and activate immune responses in plants. RpSP27 localized to the endoplasmic reticulum and triggered reactive oxygen species burst. Virus-induced gene silencing assays showed RAR1 plays an essential role in RpSP27-induced cell death in Nicotiana benthamiana. Expression analyses revealed that RpSP27 is predominantly expressed in R. pedestris salivary glands. RNA interference-mediated silencing of RpSP27 in R. pedestris significantly reduced insect survival rates and altered feeding behavior by decreasing the formation of salivary sheaths on soybeans and reducing probing and feeding duration. Furthermore, the silencing of RpSP27 in R. pedestris mitigated the staygreen syndrome in soybeans, characterized by delayed senescence and pod abnormalities. This study elucidated the role of RpSP27 in the interaction between R. pedestris and soybean, presenting a potential target for pest management strategies to protect soybean crops from the detrimental effects of R. pedestris feeding.

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臭虫唾液蛋白是昆虫取食和激活植物免疫不可或缺的物质

植食性昆虫分泌的唾液蛋白在植物与昆虫的相互作用中起着关键作用。大豆上的一种毁灭性害虫 Riptortus pedestris 的唾液蛋白 RpSP27 能诱导细胞死亡并激活植物的免疫反应，因此被选作研究对象。RpSP27 定位于内质网并引发活性氧猝灭。病毒诱导的基因沉默试验表明，RAR1 在 RpSP27 诱导的烟草细胞死亡中起着至关重要的作用。表达分析表明，RpSP27 主要在 R. pedestris 唾液腺中表达。RNA 干扰介导的 RpSP27 沉默能显著降低 R. pedestris 中昆虫的存活率，并通过减少大豆上唾液鞘的形成以及缩短探查和取食时间来改变取食行为。此外，在 R. pedestris 中沉默 RpSP27 可减轻大豆的留绿综合征，该综合征的特点是延迟衰老和豆荚异常。这项研究阐明了 RpSP27 在蓟马与大豆相互作用中的作用，为保护大豆作物免受蓟马取食的有害影响的害虫管理策略提供了一个潜在的目标。

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来源期刊

Plant, Cell & Environment 生物-植物科学

CiteScore

13.30

自引率

4.10%

发文量

253

审稿时长

1.8 months

期刊介绍： Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.