Utilizing the mutant library to investigate the functional characterization of GhGLR3.4 regulating jasmonic acid to defense pest infestation

IF 6.2 1区 生物学 Q1 PLANT SCIENCES The Plant Journal Pub Date : 2024-11-26 DOI:10.1111/tpj.17152
Qiongqiong Wang, Guangqin Yang, Ruoyu Jia, Fuqiu Wang, Guanying Wang, Zhongping Xu, Jianying Li, Bo Li, Lu Yu, Yan Zhang, Muna Alariqi, Jinglin Cao, Sijia Liang, Xianlong Zhang, Xinhui Nie, Shuangxia Jin
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Abstract

The glutamate receptor (GLR) serves as a ligand-gated ion channel that plays a vital role in plant growth, development, and stress response. Nevertheless, research on GLRs in cotton is still very limited. The present study conducted a comprehensive analysis of GLRs gene family in cotton. In total, 41 members of the GLR family were identified in cotton unveiling distinct subgroups in comparison to Arabidopsis. Among these members, the third subgroup highlights its pivotal role in cotton's defense against insect infestation. Furthermore, the CRISPR/Cas9 system was utilized to create a mutant library of GLR members, which consisted of a total of 135 independent mutant lines, resulting in the production of novel cotton materials with valuable breeding potential for pest control. Further, this study elucidates the influence of GhGLR3.4 on jasmonic acid (JA) pathway signal transduction and demonstrated its participation in the influx of intracellular Ca2+, which regulates “calcium transients” following stimulation, thereby influencing multiple intracellular reactions. The study also found that GhGLR3.4 influences the synthesis of the JA pathway and actively partakes in long-distance signal transmission among plants, facilitating the transfer of defense signals to neighbor leaves and thereby triggering systemic defense. Consequently, this research advances our knowledge of plants' comprehensive defense mechanism against insect pest infestation.

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利用突变体库研究 GhGLR3.4 调控茉莉酸防御害虫侵袭的功能特征。
谷氨酸受体(GLR)是一种配体门控离子通道,在植物的生长、发育和胁迫反应中发挥着重要作用。然而,有关棉花中 GLRs 的研究仍然非常有限。本研究对棉花中的 GLRs 基因家族进行了全面分析。与拟南芥相比,本研究在棉花中总共鉴定出 41 个 GLR 家族成员,并发现了不同的亚群。在这些成员中,第三亚群突出了其在棉花抵御虫害中的关键作用。此外,研究人员还利用 CRISPR/Cas9 系统创建了一个由 135 个独立突变株组成的 GLR 成员突变体文库,从而获得了在害虫控制方面具有育种潜力的新型棉花材料。此外,该研究还阐明了 GhGLR3.4 对茉莉酸(JA)通路信号转导的影响,并证明其参与细胞内 Ca2+ 的流入,从而调节刺激后的 "钙瞬态",进而影响细胞内的多种反应。研究还发现,GhGLR3.4 会影响 JA 途径的合成,并积极参与植物间的长距离信号传递,促进防御信号向邻近叶片的传递,从而引发系统性防御。因此,这项研究推动了我们对植物抵御虫害综合防御机制的认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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