Scanning transmission electron tomography to study virus assembly: Review for the retirement of Paul Walther.

IF 1.5 4区 工程技术 Q3 MICROSCOPY Journal of microscopy Pub Date : 2024-11-26 DOI:10.1111/jmi.13374
Susanne Wieczorek, Jacomina Krijnse Locker
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Abstract

In this short and popular review, we summarise some of our findings analysing the replication cycles of large DNA viruses using scanning transmission electron tomography (STEM tomography) that we applied in the laboratory of Paul Walther. It is also a tribute to a very kind and expert scientist, who recently retired. Transmission electron microscopy (TEM), in particular cryo-EM, has benefited tremendously from recent developments in instrumentation. However, TEM imaging remains limited by the thickness of the specimen and classical thin-section TEM typically generates 2D representations of 3D volumes. Although TEM tomography can partly overcome this limitation, the thickness of the sample, the volume that can be analysed in 3D, remains limiting. STEM tomography can partly overcome this problem, as it allows for the analysis of thicker samples, up to 1 µm in thickness. As such, it is an interesting imaging technique to analyse large DNA viruses, some of which measure 1 µm or more, and which is the focus of our research interest.

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用扫描透射电子断层扫描技术研究病毒组装:保罗-瓦尔特退休回顾。
在这篇简短而通俗的评论中,我们总结了我们在保罗-瓦尔特(Paul Walther)实验室使用扫描透射电子断层扫描技术(STEM断层扫描)分析大型DNA病毒复制周期的一些研究成果。这也是对最近退休的一位非常和蔼可亲的专家科学家的致敬。透射电子显微镜(TEM),特别是冷冻电子显微镜,从最近的仪器发展中获益匪浅。然而,透射电子显微镜成像仍然受到试样厚度的限制,传统的薄片透射电子显微镜通常生成三维体积的二维图像。虽然 TEM 层析技术可以部分克服这一限制,但样品的厚度,即可进行三维分析的体积,仍然是限制因素。STEM 层析技术可以部分克服这一问题,因为它可以分析厚度达 1 微米的较厚样品。因此,它是一种有趣的成像技术,可用于分析大型 DNA 病毒,其中一些病毒的厚度可达 1 微米或更厚,而这正是我们的研究兴趣所在。
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来源期刊
Journal of microscopy
Journal of microscopy 工程技术-显微镜技术
CiteScore
4.30
自引率
5.00%
发文量
83
审稿时长
1 months
期刊介绍: The Journal of Microscopy is the oldest journal dedicated to the science of microscopy and the only peer-reviewed publication of the Royal Microscopical Society. It publishes papers that report on the very latest developments in microscopy such as advances in microscopy techniques or novel areas of application. The Journal does not seek to publish routine applications of microscopy or specimen preparation even though the submission may otherwise have a high scientific merit. The scope covers research in the physical and biological sciences and covers imaging methods using light, electrons, X-rays and other radiations as well as atomic force and near field techniques. Interdisciplinary research is welcome. Papers pertaining to microscopy are also welcomed on optical theory, spectroscopy, novel specimen preparation and manipulation methods and image recording, processing and analysis including dynamic analysis of living specimens. Publication types include full papers, hot topic fast tracked communications and review articles. Authors considering submitting a review article should contact the editorial office first.
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