Effects of Acorns on Meat Quality and Lipid Metabolism-Related Gene Expression in Muscle Tissues of Yuxi Black Pigs.

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Metabolites Pub Date : 2024-10-26 DOI:10.3390/metabo14110578

Zhe Sun, Yadi Chang, Luyao Huang, Siyuan An, Dongyang Liu, Jinzhou Zhang, Zhiguo Miao

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Abstract

Objectives: The aim of this study was to investigate the effects of acorn diets on the composition of fatty acid (FA) and the intramuscular fat (IMF) content in Yuxi black pigs.

Methods: Ninety Yuxi black pigs with similar body weight (99.60 ± 2.32 kg) were randomly divided into five groups. The control group was fed a basal diet, and the AD20, AD30, AD40, and AD50 groups were fed experimental diets which contained 20%, 30%, 40%, and 50% acorns, respectively. The feeding experiment lasted for 120 days.

Results: The results showed that compared with the control group, the content of SFA in longissimus dorsi and biceps femoris tissues in the AD30 group decreased by 8.57% and 20.10%, and the content of MUFA increased by 5.40% and 15.83%, respectively, while the PUFA content of biceps femoris increased by 5.40% (p < 0.05). Meanwhile, the IMF content of the AD30 group was significantly higher than that of the control group in the longissimus dorsi and biceps femoris. In addition, the mRNA expression levels of the ATGL, PPARγ, and FABP4 genes in longissimus dorsi (p < 0.05) were up-regulated, and HSL were down-regulated (p < 0.05) in the AD30 group. In the biceps femoris of the AD30 group, it was observed that the expression levels of the ACC and FAS genes were up-regulated (p < 0.05), while HSL and ATGL genes were down-regulated (p < 0.05).

Conclusions: These results demonstrated that the addition of appropriate amounts of acorn to the diet (a 30% acorn diet) could improve the nutritional value of pork.

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橡子对玉溪黑猪肉质和肌肉组织中脂质代谢相关基因表达的影响

研究目的本研究旨在探讨橡子日粮对玉溪黑猪脂肪酸（FA）组成和肌内脂肪（IMF）含量的影响：将体重相近（99.60 ± 2.32 kg）的 90 头玉溪黑猪随机分为 5 组。对照组饲喂基础日粮，AD20、AD30、AD40 和 AD50 组分别饲喂含 20%、30%、40% 和 50%橡子的实验日粮。饲喂实验持续了 120 天：结果表明，与对照组相比，AD30组背阔肌和股二头肌组织中的SFA含量分别下降了8.57%和20.10%，MUFA含量分别增加了5.40%和15.83%，而股二头肌组织中的PUFA含量增加了5.40%（P＜0.05）。同时，AD30 组背阔肌和股二头肌的 IMF 含量明显高于对照组。此外，AD30组背阔肌中ATGL、PPARγ和FABP4基因的mRNA表达水平上调（p < 0.05），HSL基因的mRNA表达水平下调（p < 0.05）。在 AD30 组的股二头肌中，观察到 ACC 和 FAS 基因的表达水平上调（p < 0.05），而 HSL 和 ATGL 基因的表达水平下调（p < 0.05）：这些结果表明，在日粮中添加适量橡子（30%橡子日粮）可提高猪肉的营养价值。

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.