Comparative Assessment of the Allergenicity of Hyaluronidases from Polistes dominula (Pol d 2), Vespula vulgaris (Ves v 2), and Apis mellifera Venom (Api m 2).
Johannes Grosch, Bernadette Eberlein, Sebastian Waldherr, Mariona Pascal, Britta Dorn, Clara San Bartolomé, Federico De La Roca Pinzón, Maximilian Schiener, Ulf Darsow, Tilo Biedermann, Jonas Lidholm, Maria Beatrice Bilò, Thilo Jakob, Carsten B Schmidt-Weber, Simon Blank
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Abstract
Sensitization to cross-reactive allergens complicates identifying the culprit insect in Hymenoptera venom allergy via diagnostic tests. This study evaluates sensitization to hyaluronidases (Api m 2 from honey bee (Apis mellifera) venom, HBV; Pol d 2 from European paper wasp (Polistes dominula) venom, PDV; and Ves v 2.0101 and Ves v 2.0201 from yellow jacket (Vespula vulgaris) venom, YJV) and their cross-reactivity in allergic patients from Italy, Spain, and Germany using ImmunoCAPs, ELISA, and basophil activation tests. Sensitization rates were 45% for Api m 2 in HBV-allergic subjects, 25% for Pol d 2 in PDV-allergic individuals, and 20% and 10% for Ves v 2.0201 and Ves v 2.0101 in YJV-allergic patients, respectively. Patients primarily sensitized to Api m 2 showed minimal cross-reactivity to vespid hyaluronidases, whereas those primarily sensitized to Pol d 2 or Ves v 2.0201 exhibited IgE reactivity to Api m 2. Neither Pol d 2 nor Ves v 2.0201 triggered basophil activation. Cross-reactivity of Api m 2, Pol d 2, and Ves v 2.0201 depends on the primary sensitizing venom. Sensitization to Pol d 2 and Ves v 2.0201 remains below 25%, yet these patients may exhibit cross-reactivity to Api m 2. Conversely, HBV-allergic patients sensitized to Api m 2 show minimal reactivity to Pol d 2 or Ves v 2.0201.
对来自 Polistes dominula (Pol d 2)、Vespula vulgaris (Ves v 2) 和 Apis mellifera Venom (Api m 2) 的透明质酸酶过敏性的比较评估。
对交叉反应过敏原的过敏使通过诊断测试确定膜翅目昆虫毒液过敏的罪魁祸首变得复杂。本研究使用免疫细胞分析仪、酶联免疫吸附试验和嗜碱性粒细胞活化试验，评估了意大利、西班牙和德国过敏症患者对透明质酸酶（蜜蜂（Apis mellifera）毒液中的 Api m 2，HBV；欧洲纸蜂（Polistes dominula）毒液中的 Pol d 2，PDV；黄夹克（Vespula vulgaris）毒液中的 Ves v 2.0101 和 Ves v 2.0201，YJV）的致敏性及其交叉反应性。在对 HBV 过敏的患者中，Api m 2 的致敏率为 45%；在对 PDV 过敏的患者中，Pol d 2 的致敏率为 25%；在对 YJV 过敏的患者中，Ves v 2.0201 和 Ves v 2.0101 的致敏率分别为 20% 和 10%。主要对 Api m 2 过敏的患者对葡萄球菌透明质酸酶的交叉反应极小，而主要对 Pol d 2 或 Ves v 2.0201 过敏的患者对 Api m 2 表现出 IgE 反应。Pol d 2和Ves v 2.0201都不会引发嗜碱性粒细胞活化。Api m 2、Pol d 2 和 Ves v 2.0201 的交叉反应取决于主要致敏毒液。Pol d 2 和 Ves v 2.0201 的致敏率仍低于 25%，但这些患者可能会对 Api m 2 产生交叉反应。相反，对 Api m 2 过敏的 HBV 过敏患者对 Pol d 2 或 Ves v 2.0201 的反应性极小。
期刊介绍：
Toxins (ISSN 2072-6651) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to toxins and toxinology. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.
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