Short Term Treatment Monitoring of Renal and Inflammatory Biomarkers with Naturally Occurring Leishmaniosis: A Cohort Study of 30 Dogs.

IF 2 2区 农林科学 Q2 VETERINARY SCIENCES Veterinary Sciences Pub Date : 2024-10-22 DOI:10.3390/vetsci11110517
Valeria Pantaleo, Tommaso Furlanello, Michela Campigli, Laura Ventura, Laia Solano-Gallego
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Abstract

Various inflammatory and renal biomarkers have already been assessed for monitoring the response to anti-leishmanial therapy in canine leishmaniosis. This study assessed the parasite load, various inflammatory and renal biomarkers pre- and post-treatment, and any association between the studied variables and the degree of disease severity at diagnosis. This is a prospective cohort study of 30 client-owned dogs with leishmaniosis, classified according to LeishVet's guidelines as stage I (n = 2), stage IIa (n = 7), stage IIb (n = 6), stage III (n = 8), and stage IV (n = 7). In addition to Leishmania real-time PCR in the bone marrow, blood and urine, previously studied biomarkers, and several inflammatory and renal markers never investigated in canine leishmaniosis, such as fibrinogen, antithrombin, urinary fractional excretion of sodium, and urinary amylase-to-creatinine ratio were measured pre- and post-treatment (meglumine antimoniate or miltefosine + allopurinol). A positive Leishmania real-time PCR in the blood at diagnosis predicted a positive Leishmania real-time PCR in the bone marrow post-treatment (p = 0.003). Following treatment, antithrombin and urinary amylase-to-creatinine ratio were significantly changed (p < 0.001, respectively). Urinary amylase-to-creatinine ratio, total iron-binding capacity, and antithrombin were the variables most strongly associated with disease severity (p < 0.005, respectively). Urinary amylase-to-creatinine ratio can be a useful marker to monitor treatment response and to classify the degree of disease severity.

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自然发生的利什曼病肾脏和炎症生物标志物的短期治疗监测:30只狗的队列研究。
目前已对各种炎症和肾脏生物标志物进行了评估,以监测犬利什曼病患者对抗利什曼病疗法的反应。本研究评估了治疗前后的寄生虫量、各种炎症和肾脏生物标志物,以及研究变量与诊断时疾病严重程度之间的关联。这是一项前瞻性队列研究,研究对象是 30 只患有利什曼病的客户饲养的狗,根据 LeishVet 的指南,这些狗被分为 I 期(2 只)、IIa 期(7 只)、IIb 期(6 只)、III 期(8 只)和 IV 期(7 只)。除了检测骨髓、血液和尿液中的利什曼原虫实时 PCR 外,还检测了以前研究过的生物标志物,以及一些从未在犬利什曼病中研究过的炎症和肾脏标志物,如纤维蛋白原、抗凝血酶、尿钠排泄分数和尿淀粉酶与肌酐的比率,这些指标在治疗前和治疗后都进行了检测(甲喹抗莫那酸或米替福辛+别嘌呤醇)。诊断时血液中的利什曼原虫实时 PCR 阳性预示着治疗后骨髓中的利什曼原虫实时 PCR 阳性(p = 0.003)。治疗后,抗凝血酶和尿淀粉酶-肌酐比值发生了显著变化(分别为 p < 0.001)。尿淀粉酶与肌酐的比率、总铁结合能力和抗凝血酶是与疾病严重程度最密切相关的变量(分别为 p < 0.005)。尿淀粉酶与肌酐的比值可作为监测治疗反应和划分疾病严重程度的有用指标。
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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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