Heatwaves Increase the Polystyrene Nanoplastic-Induced Toxicity to Marine Diatoms through Interfacial Interaction Regulation

Abstract

Marine heatwaves, prolonged high-temperature extreme events in the ocean, have increased worldwide in recent decades. Plastic pollution is widespread in the ocean, and the continuous weathering of plastics leads to a substantial release of nanoplastics (NPs). However, the interactive impacts and in-depth mechanisms of heatwaves and NPs on diatoms are largely unknown. Here, we show that a heatwave intensity of 4 °C amplified the toxicity of polystyrene NPs to the globally important diatom Chaetoceros gracilis (C. gracilis), with reductions of 5.62% and 9.46% in growth rate and photosynthesis, respectively. Notably, NPs significantly inhibited the cell-specific C assimilation rate by 18.28% under heatwave conditions. The enhanced NP-induced toxicity to C. gracilis was attributed to decreased mechanical strength and increased NP adsorption under heatwave conditions, which increased membrane damage and oxidative stress. Transcriptomic analysis demonstrated that NPs disturbed redox homeostasis and caused mechanical stress to C. gracilis under heatwave conditions. Moreover, NP treatment downregulated genes (psbA and rbcL) encoding photosynthesis core proteins and the pivotal carbon-fixing enzyme RubisCo under heatwave conditions, resulting in decreased growth and C fixation rates. These findings demonstrate that heatwaves render C. gracilis susceptible to NPs and emphasize the reduced primary productivity caused by NPs under global warming.