Isoalcanivorax beigongshangi sp. nov., isolated from the fermented grains of Chinese baijiu

Abstract

A bacterial strain, REN37^T, was isolated from fermented grains of Baijiu samples collected from Sichuan, PR China. The cells of strain REN37^T was Gram-negative and aerobic. The cellular morphology exhibited rod-shaped cells without flagellum, displaying non-motility. The optimal growth condition was at 32–37 °C, pH 6.0–7.0, and with a NaCl concentration of 1–2% (w/v). Based on the analysis of 16S rRNA gene sequence, strain REN37^T was identified to belong to the genus of Isoalcanivorax. Its closest species was Isoalcanivorax pacificus W11-5 ^T (96.1%). The polar lipids were identified to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, unidentified glycolipid, two unidentified phospholipids. The predominant menaquinone was MK-12. The predominant fatty acids were C_15:0 anteiso (49.4%), C_16:0 iso (18.1%), C_17:0 anteiso (16.5%) and C_15:0 iso (9.5%). The digital DNA-DNA hybridization (dDDH), average nucleotide identity (OrthoANI) and average amino acid identify (AAI) values between strain REN37^T and its most similar species were 19.2%, 74.0% and 78.0%, respectively. The DNA G + C content of the strain REN37^T was 63.0 mol%. Three alkane 1-monooxygenase (alkB1) genes (1152 bp, 1149 bp, 1179 bp) were identified in the genome, indicating that strain REN37^T may be associated with efficient oil-degradation capabilities. Based on the results, REN37^T represents a novel specie, and the name Isoalcanivorax beigongshangi sp. nov. was proposed. The type strain is REN37^T (= GDMCC 1.3120 ^T = JCM 35319 ^T).