Correction to "Genetic and pharmacological inhibition of GITR-GITRL interaction reduces chronic lung injury induced by bleomycin instillation".

Abstract

Cuzzocrea, S., Ronchetti, S., Genovese, T., Mazzon, E., Agostini, M., Di Paola, R., Esposito, E., Muià, C., Nocentini, G. and Riccardi, C. (2007), Genetic and pharmacological inhibition of GITR-GITRL interaction reduces chronic lung injury induced by bleomycin instillation. The FASEB Journal, 21: 117-129. https://doi.org/10.1096/fj.06-6611com The authors report that while preparing Figure 10B, the beta-tubulin image was placed incorrectly; specifically, it was flipped horizontally. Due to the time that has passed, the original data are no longer available to correct the image. The figure legend has been corrected to clarify that the same beta-tubulin image was used in Figures 10A and 10B because the blots shown are from the same experiment and beta-tubulin is the experimental control. The error does not change the results or conclusions of the article. FIGURE 10 Effect of genetic or pharmacological inhibition of GITR-GITRL interaction on NF-κB activation in lungs following bleomycin administration. A basal level of IK B-e was detected in the lung tissues from sham-treated GITR^+/+ and GITR^-/-mice (A, Aa), whereas in 7 d bleomycin-treated GITR^+/+ mice IκB-α levels were substantially reduced (A, Aa). A significant prevention of IκB-é degradation induced by bleomycin instillation was observed in the lung tissues collected from GITR^-/- mice (A, Aa). Western blot with β-tubulin was performed to verify that equivalent amounts of proteins were loaded in each lane. *p ~ .05 vs. sham; °p ~ .05 vs. bleomycin-treated GITR^+/+ mice. In addition, bleomycin instillation caused a significant increase in the phosphorylation of Ser-536 in the lung tissues from GITR^+/+ mice (B, Ba). A significant reduction of the phosphorylation of p65 on Ser-536 was observed in the lung tissues from GITR^-/- mice (B, Ba). Western blot with β-tubulin was performed to verify that equivalent amounts of proteins were loaded in each lane. *p < .01 vs. sham; °p < .01 vs. bleomycin-treated -GITR^+/+ mice. Moreover, the levels of the NF-κB p65 subunit protein in the nuclear fractions from lung tissue were also significantly increased after bleomycin instillation com compared to the sham-treated mice (C, Ca). A significant reduction of the NFκB p65 protein levels was observed in the tissues from GITR^-/- mice (C, Ca). *p < .01 vs. sham; °p < .01 vs. bleomycin-treated GITR^+/+ mice. Immunoblotting in (A-C) is representative of one blot out of five analyzed. The results in (Aa, Ba, and Ca) are expressed as mean ± se from five blots. The same blot for β-tubulin was used in panels (A) and (B) because it is from the same experiment.