Curcumin alleviated dextran sulfate sodium-induced ulcerative colitis via inhibition of the Wnt/β-catenin signaling pathway and regulation of the differentiation of intestinal stem cells

IF 3.3 3区医学 Q2 PHARMACOLOGY & PHARMACY Toxicology and applied pharmacology Pub Date : 2024-11-26 DOI:10.1016/j.taap.2024.117175

Shaojie Liang , Kun Wang , Dabin Mao , Qianqian Ouyang , Xiaoping Lv , Liwei Xie , Dajian Zhu

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Abstract

In this study, we investigated the regulatory role of curcumin in the differentiation of intestinal stem cells (ISCs) in dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) model mice and explored whether this effect was mediated by the Wnt/β-catenin signaling pathway. We conducted experiments in DSS-induced UC model mice to observe changes in intestinal morphology through HE staining and detect the expression of key proteins in the Wnt/β-catenin signaling pathway. According to these findings, curcumin was found to have a significant impact on the differentiation of ISCs. These results indicated that curcumin inhibited the Wnt/β-catenin signaling pathway and restored ISC differentiation. The effects of curcumin on the Wnt/β-catenin signaling pathway were further confirmed using Wnt/β-catenin agonists. These findings provide a new perspective for understanding the behavior of ISCs in the context of inflammation and offer new insights into the development of novel therapeutic strategies and drugs for UC.

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姜黄素通过抑制Wnt/β-catenin信号通路和调节肠道干细胞分化，减轻葡聚糖硫酸钠诱导的溃疡性结肠炎

在本研究中，我们研究了姜黄素在葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎（UC）模型小鼠肠道干细胞（ISCs）分化中的调节作用，并探讨了这种作用是否通过Wnt/β-catenin信号通路介导。我们对dss诱导的UC模型小鼠进行实验，通过HE染色观察肠道形态学的变化，检测Wnt/β-catenin信号通路关键蛋白的表达。根据这些发现，姜黄素对ISCs的分化有显著影响。这些结果表明姜黄素抑制Wnt/β-catenin信号通路，恢复ISC分化。使用Wnt/β-catenin激动剂进一步证实姜黄素对Wnt/β-catenin信号通路的影响。这些发现为理解炎症背景下ISCs的行为提供了新的视角，并为UC的新治疗策略和药物的开发提供了新的见解。

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来源期刊

Toxicology and applied pharmacology 医学-毒理学

CiteScore

6.80

自引率

2.60%

发文量

309

审稿时长

32 days

期刊介绍： Toxicology and Applied Pharmacology publishes original scientific research of relevance to animals or humans pertaining to the action of chemicals, drugs, or chemically-defined natural products. Regular articles address mechanistic approaches to physiological, pharmacologic, biochemical, cellular, or molecular understanding of toxicologic/pathologic lesions and to methods used to describe these responses. Safety Science articles address outstanding state-of-the-art preclinical and human translational characterization of drug and chemical safety employing cutting-edge science. Highly significant Regulatory Safety Science articles will also be considered in this category. Papers concerned with alternatives to the use of experimental animals are encouraged. Short articles report on high impact studies of broad interest to readers of TAAP that would benefit from rapid publication. These articles should contain no more than a combined total of four figures and tables. Authors should include in their cover letter the justification for consideration of their manuscript as a short article.