Identifying the active antioxidant ingredients in Arisaema Cum Bile aqueous extract on the basis of spectrum-effect relationships

Abstract

At present, the research on Arisaema Cum Bile mainly focuses on the analysis of its components and changes in pharmacological effects. The chemical composition of the water extract, the material basis and mechanism of its anti-oxidation and anti-febrile convulsion are still unclear. In this study, fingerprint-pharmacological relationships were used to determine the pharmacodynamic and pharmacological material basis of the antioxidant effects of Arisaema Cum Bile aqueous extract.

Methods

The High Performance Liquid Chromatography (HPLC) fingerprint of the aqueous extract of Arisaema Cum Bile was established by determining the common peaks, recording the peak areas and evaluating the similarities between different batches. Moreover, the antioxidant capacity of each batch of processed Arisaema Cum Bile extracts was determined, and spectrum-effect relationship analysis and screening were conducted on the basis of the fingerprint. The antioxidant activities of the Arisaema Cum Bile aqueous extract ingredients were verified by evaluating the isolated compounds.

Results

The HPLC fingerprints of 15 batches of aqueous extracts of Arisaema Cum Bile aqueous extracts were established via ultraviolet (UV) and evaporative light scattering detection methods. There was a total of 22 common peaks, 11 of which were identified as uracil, hypoxanthine, xanthine, uridine, guanosine, adenosine, shaftoside, isoschaftoside, taurohyodeoxycholic acid, taurochenodeoxycholic acid and glycinechenodeoxycholic acid. Spectrum-effect relationship analysis revealed that shaftoside, isoschaftoside, taurochenodeoxycholic acid and glycochenodeoxycholic acid may be the active antioxidant ingredients in this extract, which was verified since all four ingredients displayed dose-dependent antioxidant effects when tested individually.

Conclusion

A fingerprint of the aqueous extract of Arisaema Cum Bile aqueous extract was established, the antioxidant capacities of the extracts were measured, and the active antioxidant ingredients were identified through spectrum-effect relationship analysis.