Protective effects of puerarin combined with bone marrow mesenchymal stem cells on nerve injury in rats with ischemic stroke.

IF 1.5 4区医学 Q4 NEUROSCIENCES Brain injury Pub Date : 2024-11-28 DOI:10.1080/02699052.2024.2433667

Jiane Chen, Xiaoli Wu, Dongliang Nie, Zhimin Yu

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Abstract

Background: Bone marrow mesenchymal stem cells (BM-MSCs) transplantation shows promise for treating ischemic stroke, but the ischemic environment that follows cerebral infarction hinders the survival of transplanted cells. We aimed to study the effects of puerarin (Pue) in combination with BM-MSCs on cerebral ischemic injury.

Methods: After middle cerebral artery occlusion (MCAO) models were prepared by suture-occluded method, rats were randomly allocated to the sham, MCAO, Pue (50 mg/kg), BM-MSCs (2×106), and BM-MSCs+Pue groups. The neurological function, infarct area, levels of inflammation-related factors, brain tissue damage, apoptosis, BrdU, Beclin1, and LC3 levels were then assessed.

Results: Pue and BM-MSCs reduced the modified neurological severity score, cerebral infarction area, and serum inflammation-related factor levels for MCAO rats. Furthermore, Pue and BM-MSCs interventions ameliorated brain tissue damage, and repressed apoptosis of brain tissues in MCAO rats. Moreover, Pue or BM-MSCs enhanced BrdU expression, restrained LC3II/LC3I ratio and Beclin 1 expression in MCAO rats' brain tissues. Importantly, the combination of Pue and BM-MSCs exhibited more pronounced effects on aforementioned outcomes.

Conclusion: The combination of Pue and BM-MSCs facilitated the recovery of neurological function in rats after cerebral ischemic damage, and the mechanisms may correlate with the repression of neuronal apoptosis, inflammation, and autophagy.

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葛根素联合骨髓间充质干细胞对缺血性脑卒中大鼠神经损伤的保护作用。

背景：骨髓间充质干细胞（BM-MSCs）移植有望治疗缺血性卒中，但脑梗死后的缺血环境阻碍了移植细胞的存活。我们旨在研究葛根素（Pue）联合骨髓间充质干细胞对脑缺血损伤的影响。方法：采用缝合法制备大脑中动脉闭塞（MCAO）模型后，将大鼠随机分为假手术组、MCAO组、Pue组（50 mg/kg）、BM-MSCs组（2×106）和BM-MSCs+Pue组。然后评估神经功能、梗死面积、炎症相关因子水平、脑组织损伤、细胞凋亡、BrdU、Beclin1和LC3水平。结果：Pue和BM-MSCs降低了MCAO大鼠的改良神经系统严重程度评分、脑梗死面积和血清炎症相关因子水平。此外，Pue和BM-MSCs干预可以改善MCAO大鼠的脑组织损伤，抑制脑组织凋亡。此外，Pue或BM-MSCs增强了MCAO大鼠脑组织中BrdU的表达，抑制了LC3II/LC3I比值和Beclin 1的表达。重要的是，Pue和BM-MSCs联合使用对上述结果的影响更为明显。结论：Pue联合BM-MSCs可促进大鼠脑缺血损伤后神经功能的恢复，其机制可能与抑制神经元凋亡、炎症和自噬有关。

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Pue

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TTC staining kits

来源期刊

Brain injury 医学-康复医学

CiteScore

3.50

自引率

5.30%

发文量

148

审稿时长

12 months

期刊介绍： Brain Injury publishes critical information relating to research and clinical practice, adult and pediatric populations. The journal covers a full range of relevant topics relating to clinical, translational, and basic science research. Manuscripts address emergency and acute medical care, acute and post-acute rehabilitation, family and vocational issues, and long-term supports. Coverage includes assessment and interventions for functional, communication, neurological and psychological disorders.