Multifunctional metabolites of Streptomyces kunmingensis BS19 from bamboo rhizosphere soil.

IF 2.3 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY International Microbiology Pub Date : 2024-11-29 DOI:10.1007/s10123-024-00619-2

Murthy Sangeetha, Sivakumar Sasirekha, Jaganathan Mahendran, Anbalmani Sivarajan, Manikkam Radhakrishnan, Kaari Manigundan, Syed G Dastager, Singaravel Sengottuvelu, Ramasamy Balagurunathan

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Abstract

A pigmented bioactive molecule from Streptomyces kunmingensis BS19 was isolated, characterized, and evaluated for anti-infective, antiproliferative, and wound-healing properties. The yellow-red pigment produced from the strain BS19 showed promising activity against methicillin-resistant Staphylococcus aureus (MRSA) and Mycobacterium tuberculosis. Extracellular bioactive pigment from the strain BS19 was produced by agar surface fermentation and purified through bioassay guided preparative HPLC-based purification. Based on the results of UV, FT-IR, GC-MS, ¹H NMR, and ¹³C NMR spectral analyses, the purified pigment was identified as a chromopeptide class of molecule with phenoxazinone chromophore. Its molecular weight was determined as the chemical formula C₆₄H₉₀N₁₂O₁₆ and molecular weight 1283 g/mol. It exhibited promising antimicrobial activity against Staphylococcus aureus (ATCC 1720) and antiproliferative activity against 14 types of human cancer cell lines. It showed good in vivo wound-healing activity in the rat model. The present study explored Streptomyces kunmingensis as a newly added source for the isolation of chromopeptide antibiotics for antimicrobial, wound-healing, and anticancer applications.

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竹根际土壤中昆明链霉菌BS19的多功能代谢产物

从昆明链霉菌BS19中分离得到一种色素活性分子，并对其抗感染、抗增殖和伤口愈合性能进行了表征和评价。菌株BS19产生的黄红色色素对耐甲氧西林金黄色葡萄球菌（MRSA）和结核分枝杆菌具有良好的抗氧化活性。采用琼脂表面发酵法制备菌株BS19的胞外活性色素，并采用生物测定指导制备高效液相色谱法纯化。通过紫外、红外光谱、气相色谱-质谱、核磁共振氢谱和核磁共振13C谱分析，鉴定该色素为具有吩恶嗪酮类发色团的色肽类分子。测定其分子量为化学式C64H90N12O16，分子量为1283 g/mol。对金黄色葡萄球菌（ATCC 1720）具有良好的抑菌活性，对14种人类癌细胞具有抗增殖活性。在大鼠模型中表现出良好的体内创面愈合活性。本研究探索了昆明链霉菌作为一种新的来源，用于抗菌、创面愈合和抗癌等方面的抗菌肽的分离。

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来源期刊

International Microbiology 生物-生物工程与应用微生物

CiteScore

5.50

自引率

3.20%

发文量

审稿时长

3 months

期刊介绍： International Microbiology publishes information on basic and applied microbiology for a worldwide readership. The journal publishes articles and short reviews based on original research, articles about microbiologists and their work and questions related to the history and sociology of this science. Also offered are perspectives, opinion, book reviews and editorials. A distinguishing feature of International Microbiology is its broadening of the term microbiology to include eukaryotic microorganisms.