Pub Date : 2024-11-18DOI: 10.1007/s10123-024-00616-5
Samira Pakdel, Ali Beheshti Ale Agha, Rouhallah Sharifi, Alireza Habibi, Firoozeh Gholami
Relying on native microorganisms is crucial for bioremediating petroleum-contaminated soils within this oil field. This study aimed to isolate native bacteria and investigate their ability to degrade petroleum hydrocarbons in contaminated soils. Flame ionization detector gas chromatography analyzed the capacity of Indigenous bacterial isolates to break down diesel fuel in an aquatic environment. Soil samples were collected from the Naft-Shahr area. Initially, 126 bacterial isolates were obtained from these soils, of which only 48 species could grow on a diesel-containing medium. Further analysis identified the top 8 isolates with high diesel removal potential. Results showed that the diesel removal percentage ranged from 26 to 76% at an initial diesel concentration of 3.7 g. L - 1 after 48 h, without adding any supplementary surface-active agent. Four top isolates were selected based on their degradation activity, removal yield, and biodegradation rate and were identified using 16S rRNA gene sequencing and phylogenetic analysis. Sequence alignment revealed that isolates B11Pet, B19Pet, B27Pet, and B48Pet belong to Staphylococcus gallinarum, Paenarthrobacter nitroguajacolicus, Arthrobacter citreus, and Bacillus thuringiensis, respectively. Among these, Bacillus thuringiensis (B48Pet), with a specific growth rate of 0.211 h⁻1, could uniformly remove all diesel hydrocarbon fractions at 58.81 mg. L⁻1. h⁻1. This strain, alone or in consortia, represents a promising strategy for the bioremediation of petroleum-contaminated soils.
{"title":"Diesel-degradation by indigenous bacteria of petroleum-contaminated soils.","authors":"Samira Pakdel, Ali Beheshti Ale Agha, Rouhallah Sharifi, Alireza Habibi, Firoozeh Gholami","doi":"10.1007/s10123-024-00616-5","DOIUrl":"https://doi.org/10.1007/s10123-024-00616-5","url":null,"abstract":"<p><p>Relying on native microorganisms is crucial for bioremediating petroleum-contaminated soils within this oil field. This study aimed to isolate native bacteria and investigate their ability to degrade petroleum hydrocarbons in contaminated soils. Flame ionization detector gas chromatography analyzed the capacity of Indigenous bacterial isolates to break down diesel fuel in an aquatic environment. Soil samples were collected from the Naft-Shahr area. Initially, 126 bacterial isolates were obtained from these soils, of which only 48 species could grow on a diesel-containing medium. Further analysis identified the top 8 isolates with high diesel removal potential. Results showed that the diesel removal percentage ranged from 26 to 76% at an initial diesel concentration of 3.7 g. L - <sup>1</sup> after 48 h, without adding any supplementary surface-active agent. Four top isolates were selected based on their degradation activity, removal yield, and biodegradation rate and were identified using 16S rRNA gene sequencing and phylogenetic analysis. Sequence alignment revealed that isolates B11Pet, B19Pet, B27Pet, and B48Pet belong to Staphylococcus gallinarum, Paenarthrobacter nitroguajacolicus, Arthrobacter citreus, and Bacillus thuringiensis, respectively. Among these, Bacillus thuringiensis (B48Pet), with a specific growth rate of 0.211 h⁻<sup>1</sup>, could uniformly remove all diesel hydrocarbon fractions at 58.81 mg. L⁻<sup>1</sup>. h⁻<sup>1</sup>. This strain, alone or in consortia, represents a promising strategy for the bioremediation of petroleum-contaminated soils.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-15DOI: 10.1007/s10123-024-00614-7
Diem Hong Tran, Hau Thi Tran, Binh Thi Tu Vo, Le Minh Bui, Huong Thi Thu Phung
Vibrio parahaemolyticus in seafood and marine environments poses significant health risks, causing gastroenteritis worldwide. Current detection methods fail to differentiate live from dead cells, leading to inaccuracies in food safety assessments. This study introduces a novel method combining cis-diamminedichloroplatinum (CDDP) with direct colorimetric loop-mediated isothermal amplification (LAMP) for rapid and accurate detection of viable V. parahaemolyticus cells in seafood samples. CDDP treatment at 37 °C for 30 min selectively inhibits DNA from dead cells, enhancing the specificity of the assay by ensuring only live cell DNA is amplified. The optimized CDDP-LAMP procedure detects alive V. parahaemolyticus within 1 h, with results observable through a color change. The CDDP-LAMP assay demonstrates excellent specificity, identifying live V. parahaemolyticus cells while excluding dead cells and other bacteria. It shows a detection limit of 2.348 CFU per reaction and successfully detects V. parahaemolyticus in seafood samples across different food matrices. This study is the first to combine CDDP with colorimetric LAMP for direct detecting viable bacteria in food, enhancing specificity by eliminating signals from dead cells. The CDDP-LAMP assay provides a rapid, accurate process for detecting viable V. parahaemolyticus cells, especially in resource-limited settings. It also gives a model for screening different bacterial pathogens, speeding up and improving foodborne illness risk assessments.
{"title":"Rapid on-site detection of viable Vibrio parahaemolyticus in seafood using cis-diamminedichloroplatinum and colorimetric loop-mediated isothermal amplification (CDDP-LAMP).","authors":"Diem Hong Tran, Hau Thi Tran, Binh Thi Tu Vo, Le Minh Bui, Huong Thi Thu Phung","doi":"10.1007/s10123-024-00614-7","DOIUrl":"https://doi.org/10.1007/s10123-024-00614-7","url":null,"abstract":"<p><p>Vibrio parahaemolyticus in seafood and marine environments poses significant health risks, causing gastroenteritis worldwide. Current detection methods fail to differentiate live from dead cells, leading to inaccuracies in food safety assessments. This study introduces a novel method combining cis-diamminedichloroplatinum (CDDP) with direct colorimetric loop-mediated isothermal amplification (LAMP) for rapid and accurate detection of viable V. parahaemolyticus cells in seafood samples. CDDP treatment at 37 °C for 30 min selectively inhibits DNA from dead cells, enhancing the specificity of the assay by ensuring only live cell DNA is amplified. The optimized CDDP-LAMP procedure detects alive V. parahaemolyticus within 1 h, with results observable through a color change. The CDDP-LAMP assay demonstrates excellent specificity, identifying live V. parahaemolyticus cells while excluding dead cells and other bacteria. It shows a detection limit of 2.348 CFU per reaction and successfully detects V. parahaemolyticus in seafood samples across different food matrices. This study is the first to combine CDDP with colorimetric LAMP for direct detecting viable bacteria in food, enhancing specificity by eliminating signals from dead cells. The CDDP-LAMP assay provides a rapid, accurate process for detecting viable V. parahaemolyticus cells, especially in resource-limited settings. It also gives a model for screening different bacterial pathogens, speeding up and improving foodborne illness risk assessments.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-14DOI: 10.1007/s10123-024-00609-4
Gehad A El-Nahas, Mohsen E Ibrahim, Zakaria A M Baka, Ali H Ibrahim
Drought constitutes a significant abiotic stressor that hinders plant growth and productivity in many countries. Habitat-adapted endophytic fungi offer an environmentally sustainable approach to address this issue by promoting plant development and enhancing resilience against abiotic stresses. In this study, 30 endophytic fungal isolates were recovered from some wild plants in the extreme habitats of Port Said Governorate, Egypt, and evaluated for their drought tolerance using polyethylene glycol (PEG-6000). Only eight isolates demonstrated drought tolerance properties and were further evaluated for their plant growth-promoting biochemical activities and ability to improve maize germination under simulated drought conditions. All eight isolates exhibited enzyme activity for endo-1,4-β-glucanase, amylase, and pectinase, and most displayed significant nutrient mobilization, with siderophores production ranging from 4 to 89%, ammonia production from 1 to 7 μmol/ml, and phosphate solubilization from 129 to 256 µg/ml. Additionally, all isolates showed strong antioxidant activity and high total phenolic content, with some also producing notable levels of indole acetic acid (IAA) and gibberellic acid (GA3) as plant growth hormones. Coating maize grains with spore suspensions of the eight fungal isolates, in general, significantly increased their germination parameters and seedling vigor in vitro under 8% PEG-6000. This enhancement was particularly pronounced with Neurospora sitophila (P8L4M1) and Penicillium tardochrysogenum (P15L4M1), which increased the vigor of maize seedlings by approximately 308% compared to untreated control. Molecular identification of P8L4M1 and P15L4M1 was performed by amplifying the 28S rRNA gene. This study disclosed unique endophytic fungal isolates with promising potential for enhancing drought resistance in maize.
{"title":"Scrutinizing harsh habitats endophytic fungi and their prospective effect on water-stressed maize seedlings.","authors":"Gehad A El-Nahas, Mohsen E Ibrahim, Zakaria A M Baka, Ali H Ibrahim","doi":"10.1007/s10123-024-00609-4","DOIUrl":"https://doi.org/10.1007/s10123-024-00609-4","url":null,"abstract":"<p><p>Drought constitutes a significant abiotic stressor that hinders plant growth and productivity in many countries. Habitat-adapted endophytic fungi offer an environmentally sustainable approach to address this issue by promoting plant development and enhancing resilience against abiotic stresses. In this study, 30 endophytic fungal isolates were recovered from some wild plants in the extreme habitats of Port Said Governorate, Egypt, and evaluated for their drought tolerance using polyethylene glycol (PEG-6000). Only eight isolates demonstrated drought tolerance properties and were further evaluated for their plant growth-promoting biochemical activities and ability to improve maize germination under simulated drought conditions. All eight isolates exhibited enzyme activity for endo-1,4-β-glucanase, amylase, and pectinase, and most displayed significant nutrient mobilization, with siderophores production ranging from 4 to 89%, ammonia production from 1 to 7 μmol/ml, and phosphate solubilization from 129 to 256 µg/ml. Additionally, all isolates showed strong antioxidant activity and high total phenolic content, with some also producing notable levels of indole acetic acid (IAA) and gibberellic acid (GA<sub>3</sub>) as plant growth hormones. Coating maize grains with spore suspensions of the eight fungal isolates, in general, significantly increased their germination parameters and seedling vigor in vitro under 8% PEG-6000. This enhancement was particularly pronounced with Neurospora sitophila (P8L4M1) and Penicillium tardochrysogenum (P15L4M1), which increased the vigor of maize seedlings by approximately 308% compared to untreated control. Molecular identification of P8L4M1 and P15L4M1 was performed by amplifying the 28S rRNA gene. This study disclosed unique endophytic fungal isolates with promising potential for enhancing drought resistance in maize.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-13DOI: 10.1007/s10123-024-00613-8
Syed Ata Ur Rahman Shah, Bin Tang, Dekui He, Yujiang Hao, Maaz Ahmad, Ghulam Nabi, Richard McLaughlin, Chaoqun Wang, Zhangbing Kou, Kexiong Wang
Social separation, or the absence of social support, can cause physical and psychological health issues. Social separation is crucial for the welfare of the Yangtze finless porpoise (YFP) in captivity because they face many challenges like frequent social separation, noise from visitors, and animal replacement, which can cause psychological and physiological stress. This research is aimed at assessing the potential negative impacts of social separation on the gut microbiome and metabolome of captive YFP, focusing on the potential imbalances caused by mother-calf separation. The study found that social separation did not alter the alpha and beta diversity of the gut microbes but increased the abundance of disease-associated taxa such as Romboutsia, Terrisporobacter, and Clostridium_sensu_stricto_13 in the MC (mother-calf) group while increasing Paeniclostridium and Clostridium_sensu_stricto_1 associated with host health in the MS (mother-separated) group. The fecal metabolome underwent significant changes during social separation, with stress-associated metabolites like kainic acid, phenethylamine glucuronide, and paxilline upregulated in the MC group and host health-associated metabolites like butyric acid, 6-hydroxyhexanoic acid, and fosinopril downregulated in the MS group. In addition, there was a strong association between the fecal microbiome and the metabolome of captive YFPs. The study enhances our comprehension of the detrimental effects of social separation, which result in disruptions in the gut microbiome and fecal metabolome. The study is aimed at introducing a new method for assessing the health and welfare of endangered mammals in captivity.
社会分离或缺乏社会支持会导致生理和心理健康问题。社会分离对圈养长江江豚的福利至关重要,因为它们面临着许多挑战,如频繁的社会分离、游客的噪音和动物替代,这些都会造成心理和生理压力。本研究旨在评估社会分离对人工饲养的长江江豚肠道微生物组和代谢组的潜在负面影响,重点关注母豚分离可能导致的失衡。研究发现,社会分离并没有改变肠道微生物的α和β多样性,但在MC(母-犊)组中增加了与疾病相关的类群,如Romboutsia、Terrisporobacter和Clostridium_sensu_stricto_13,而在MS(母-犊分离)组中增加了与宿主健康相关的Paeniclostridium和Clostridium_sensu_stricto_1。在社会分离过程中,粪便代谢组发生了显著变化,MC 组凯尼酸、苯乙胺葡萄糖醛酸苷和帕西林等与应激相关的代谢物上调,而 MS 组丁酸、6-羟基己酸和福辛普利等与宿主健康相关的代谢物下调。此外,圈养 YFP 的粪便微生物组和代谢组之间也存在密切联系。这项研究加深了我们对社会分离造成肠道微生物组和粪便代谢组紊乱的有害影响的理解。该研究旨在为评估圈养濒危哺乳动物的健康和福利引入一种新方法。
{"title":"Effect of calf separation on gut microbiome and fecal metabolome of mother in the captive Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis).","authors":"Syed Ata Ur Rahman Shah, Bin Tang, Dekui He, Yujiang Hao, Maaz Ahmad, Ghulam Nabi, Richard McLaughlin, Chaoqun Wang, Zhangbing Kou, Kexiong Wang","doi":"10.1007/s10123-024-00613-8","DOIUrl":"https://doi.org/10.1007/s10123-024-00613-8","url":null,"abstract":"<p><p>Social separation, or the absence of social support, can cause physical and psychological health issues. Social separation is crucial for the welfare of the Yangtze finless porpoise (YFP) in captivity because they face many challenges like frequent social separation, noise from visitors, and animal replacement, which can cause psychological and physiological stress. This research is aimed at assessing the potential negative impacts of social separation on the gut microbiome and metabolome of captive YFP, focusing on the potential imbalances caused by mother-calf separation. The study found that social separation did not alter the alpha and beta diversity of the gut microbes but increased the abundance of disease-associated taxa such as Romboutsia, Terrisporobacter, and Clostridium_sensu_stricto_13 in the MC (mother-calf) group while increasing Paeniclostridium and Clostridium_sensu_stricto_1 associated with host health in the MS (mother-separated) group. The fecal metabolome underwent significant changes during social separation, with stress-associated metabolites like kainic acid, phenethylamine glucuronide, and paxilline upregulated in the MC group and host health-associated metabolites like butyric acid, 6-hydroxyhexanoic acid, and fosinopril downregulated in the MS group. In addition, there was a strong association between the fecal microbiome and the metabolome of captive YFPs. The study enhances our comprehension of the detrimental effects of social separation, which result in disruptions in the gut microbiome and fecal metabolome. The study is aimed at introducing a new method for assessing the health and welfare of endangered mammals in captivity.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
It is vital that we monitor the gut microbiota of sentinel species such as spotted seals (Phoca largha) and their association with habitat microbiomes, which can provide critical data for assessing the health of marine mammals and their potential ecological influences. In this study, PacBio technology was used to sequence the full-length bacterial 16S rRNA gene from the feces of captive and wild spotted seals, as well as samples from a wild population and their habitats. Based on the pathogen identification results, the gut microbiota of wild and captive spotted seals showed similar levels of pathogen richness and abundance. In particular, the pathogen profiles in wild spotted seals were more variable, with a high risk of disease in a minority of individuals. Meanwhile, the gut microbiota of spotted seals was significantly less diverse than their habitat microbiomes. Firmicutes and Proteobacteria dominated the gut microbiota of spotted seals and their habitat microbiomes, respectively. Furthermore, network analysis revealed that the gut microbiota of spotted seals was simple and weak. The ratios of microbial turnover between spotted seal gut microbiota and their habitat microbiomes were further analyzed using SourceTracker, and the estimated values were low (< 0.1%). These results provide baseline data on pathogen profiles in spotted seals and their potential interactions with habitat microbiomes.
{"title":"Unstable pathogen profile in spotted seal (Phoca largha) gut microbiota and limited turnover with habitat microbiome.","authors":"Jing Du, Zhen Wang, Xianggang Gao, Yankuo Xing, Zhichuang Lu, Duohui Li, Edmond Sanganyado, Jiashen Tian","doi":"10.1007/s10123-024-00615-6","DOIUrl":"https://doi.org/10.1007/s10123-024-00615-6","url":null,"abstract":"<p><p>It is vital that we monitor the gut microbiota of sentinel species such as spotted seals (Phoca largha) and their association with habitat microbiomes, which can provide critical data for assessing the health of marine mammals and their potential ecological influences. In this study, PacBio technology was used to sequence the full-length bacterial 16S rRNA gene from the feces of captive and wild spotted seals, as well as samples from a wild population and their habitats. Based on the pathogen identification results, the gut microbiota of wild and captive spotted seals showed similar levels of pathogen richness and abundance. In particular, the pathogen profiles in wild spotted seals were more variable, with a high risk of disease in a minority of individuals. Meanwhile, the gut microbiota of spotted seals was significantly less diverse than their habitat microbiomes. Firmicutes and Proteobacteria dominated the gut microbiota of spotted seals and their habitat microbiomes, respectively. Furthermore, network analysis revealed that the gut microbiota of spotted seals was simple and weak. The ratios of microbial turnover between spotted seal gut microbiota and their habitat microbiomes were further analyzed using SourceTracker, and the estimated values were low (< 0.1%). These results provide baseline data on pathogen profiles in spotted seals and their potential interactions with habitat microbiomes.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-18DOI: 10.1007/s10123-024-00604-9
Jingya Qian, Dazhou Lu, Zixuan Zhang, Di Chen, Feng Zhao, Shuhao Huo, Feng Wang, Haile Ma, Juan Kan
The objective of this study was to investigate the effect of low-frequency alternating magnetic field (LF-AMF) on the production of extracellular polysaccharide (EPS) by submerged fermentation of Pleurotus citrinopileatus. The fermentation conditions optimized by the central composite design method were as follows: fermentation time of 6.18 days, temperature of 28.28 °C, shaking speed of 149.04 r/min, and inoculum amount of 8.43%. Under these conditions, a LF-AMF was applied to the submerged fermentation of P. citrinopileatus. When the intensity of LF-AMF was 40 Gs, the initial intervention time was 24 h after inoculation, and the treatment time was 6 h at one time, the mycelial biomass of P. citrinopileatus increased by 11.30%, and the EPS yield increased by 23.09% compared with the fermentation without LF-AMF treatment. The morphology of mycelium after LF-AMF treatment was observed by scanning electron microscopy. It was found that the surface of mycelium was wrinkled, and the structure of mycelium was loose, which might be more conducive to the production of EPS. Mycelium diameter decreased, and ATPase activity increased, indicating that LF-AMF had a positive effect on the production of EPS by P. citrinopileatus fermentation. Moreover, LF-AMF could improve the permeability of the mycelial cell membrane, facilitate the exchange of intracellular and extracellular substances, and increase the metabolic capacity of P. citrinopileatus. In vitro antioxidant test of EPS showed that LF-AMF treatment also improved its antioxidant capacity.
{"title":"Effect of low-frequency alternating magnetic field on exopolysaccharide production and antioxidant capacity of Pleurotus citrinopileatus by submerged fermentation.","authors":"Jingya Qian, Dazhou Lu, Zixuan Zhang, Di Chen, Feng Zhao, Shuhao Huo, Feng Wang, Haile Ma, Juan Kan","doi":"10.1007/s10123-024-00604-9","DOIUrl":"https://doi.org/10.1007/s10123-024-00604-9","url":null,"abstract":"<p><p>The objective of this study was to investigate the effect of low-frequency alternating magnetic field (LF-AMF) on the production of extracellular polysaccharide (EPS) by submerged fermentation of Pleurotus citrinopileatus. The fermentation conditions optimized by the central composite design method were as follows: fermentation time of 6.18 days, temperature of 28.28 °C, shaking speed of 149.04 r/min, and inoculum amount of 8.43%. Under these conditions, a LF-AMF was applied to the submerged fermentation of P. citrinopileatus. When the intensity of LF-AMF was 40 Gs, the initial intervention time was 24 h after inoculation, and the treatment time was 6 h at one time, the mycelial biomass of P. citrinopileatus increased by 11.30%, and the EPS yield increased by 23.09% compared with the fermentation without LF-AMF treatment. The morphology of mycelium after LF-AMF treatment was observed by scanning electron microscopy. It was found that the surface of mycelium was wrinkled, and the structure of mycelium was loose, which might be more conducive to the production of EPS. Mycelium diameter decreased, and ATPase activity increased, indicating that LF-AMF had a positive effect on the production of EPS by P. citrinopileatus fermentation. Moreover, LF-AMF could improve the permeability of the mycelial cell membrane, facilitate the exchange of intracellular and extracellular substances, and increase the metabolic capacity of P. citrinopileatus. In vitro antioxidant test of EPS showed that LF-AMF treatment also improved its antioxidant capacity.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142465463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-16DOI: 10.1007/s10123-024-00608-5
Jeimy L Valbuena-Rodríguez, Ingrid Fonseca-Guerra, Claudia Buitrago-Yomayusa, Alexander Puentes-S, Martha Elizabeth Benavides Rozo
Chenopodium quinoa, globally recognized as quinoa, stands out as one of the cereals with the highest nutritional value native to the Americas. It is cultivated in the Andes Mountain range, and Colombia is no exception, with the Boyacá department emerging as a significant quinoa-producing region. The quinoa ecosystem harbors a rich array of microorganisms within its rhizosphere. In this current study, nitrogen-fixing and phosphate-solubilizing isolates AM-0261 (Pantoea ananatis) and AM-0263 (Pantoea agglomerans) were sourced from rhizospheric soil samples of quinoa. These isolates were subjected to biochemical characterization and identification through PCR analysis and Sanger sequencing targeting a partial sequence of the 16 s region of the rRNA. To assess their potential as plant growth-promoting rhizobacteria (PGPR), taking into consideration that P. ananatis is an IAA producer, greenhouse-based bioassays were conducted using seedlings. Additionally, dual culture assays were employed to showcase their antagonistic capabilities against primary beneficial and phytopathogenic fungi associated with quinoa cultivation in the region. The results underscore the remarkable potential of P. ananatis as a PGPR and a biocontrol agent against quinoa's phytopathogenic fungi. This study represents the pioneering exploration of the interaction between these two bacterial strains with quinoa rhizosphere tissue. In addition, the isolate of P. annatis (AM-0261) stands out, which presents phosphate solubilization capacity, nitrogen fixation, antagonistic capacity, and IAA production, characteristics that make it a promising strain for its use for the management of diseases of fungal origin, and in the future, it could be useful in reducing the use of chemical fertilizers.
{"title":"Isolation and characterization of Pantoea ananatis and P. agglomerans in quinoa: P. ananatis as a potential fungal biocontroller and plant growth promoter.","authors":"Jeimy L Valbuena-Rodríguez, Ingrid Fonseca-Guerra, Claudia Buitrago-Yomayusa, Alexander Puentes-S, Martha Elizabeth Benavides Rozo","doi":"10.1007/s10123-024-00608-5","DOIUrl":"https://doi.org/10.1007/s10123-024-00608-5","url":null,"abstract":"<p><p>Chenopodium quinoa, globally recognized as quinoa, stands out as one of the cereals with the highest nutritional value native to the Americas. It is cultivated in the Andes Mountain range, and Colombia is no exception, with the Boyacá department emerging as a significant quinoa-producing region. The quinoa ecosystem harbors a rich array of microorganisms within its rhizosphere. In this current study, nitrogen-fixing and phosphate-solubilizing isolates AM-0261 (Pantoea ananatis) and AM-0263 (Pantoea agglomerans) were sourced from rhizospheric soil samples of quinoa. These isolates were subjected to biochemical characterization and identification through PCR analysis and Sanger sequencing targeting a partial sequence of the 16 s region of the rRNA. To assess their potential as plant growth-promoting rhizobacteria (PGPR), taking into consideration that P. ananatis is an IAA producer, greenhouse-based bioassays were conducted using seedlings. Additionally, dual culture assays were employed to showcase their antagonistic capabilities against primary beneficial and phytopathogenic fungi associated with quinoa cultivation in the region. The results underscore the remarkable potential of P. ananatis as a PGPR and a biocontrol agent against quinoa's phytopathogenic fungi. This study represents the pioneering exploration of the interaction between these two bacterial strains with quinoa rhizosphere tissue. In addition, the isolate of P. annatis (AM-0261) stands out, which presents phosphate solubilization capacity, nitrogen fixation, antagonistic capacity, and IAA production, characteristics that make it a promising strain for its use for the management of diseases of fungal origin, and in the future, it could be useful in reducing the use of chemical fertilizers.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142465464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bacteria have two trophic lifestyles in aquatic ecosystems, i.e., free-living (FL) and particle-attached (PA), with different but essential ecological roles. However, relevant knowledge is still dearth in the upstream source region of the Himalayan Rivers. Thus, we emphasized a comparative study on community composition, co-occurrence, and geographic distribution of the FL and PA bacteria and the effect of environmental factors in the source region of the Ganges and Brahmaputra Rivers. PA bacteria relative to FL harbored a significantly higher local diversity, richness, and evenness. A significantly higher abundance of Betaproteobacteria, Verrucomicrobiota, and Planctomycetota in PA trophic lifestyle and Gammaproteobacteria and Actinomycetota in FL tropic lifestyle and indicator OTUs belonging to related taxa were observed. The spatial variation of the FL and PA bacterial communities was most significantly impacted by dispersal limitation as a discrete factor. Among the environmental parameters, the total nitrogen (TN) was found to be a significant (P < 0.001) driver of the variation in PA communities. Meanwhile, particulate organic carbon (POC) and TN considerably explained the variation of FL communities. A significant correlation (P < 0.001) of TN with dominant bacterial taxa (Pseudomonadota, Actinomycetota, and Verrucomicrobiota) and FL and PA indicator OTUs associated with these taxa further confirmed nitrogen as the limiting nutrient in the source region of the Ganges and Brahmaputra Rivers. The co-occurrence network topological characteristics showed that the PA network was more stable than the FL network, which was more complicated and unstable. Thus, it can be speculated that FL communities relative to PA are more vulnerable to shifting upon disturbances.
细菌在水生生态系统中有两种营养生活方式,即自由生活(FL)和颗粒附着(PA),它们发挥着不同但重要的生态作用。然而,喜马拉雅山脉河流上游源头地区的相关知识仍然匮乏。因此,我们着重比较研究了恒河和雅鲁藏布江源头地区 FL 和 PA 细菌的群落组成、共存情况、地理分布以及环境因素的影响。与 FL 细菌相比,PA 细菌在当地的多样性、丰富度和均匀度明显更高。在 PA 营养级生活方式中,Betaproteobacteria、Verrucomicrobiota 和 Planctomycetota 的丰度明显更高;在 FL 营养级生活方式中,Gammaproteobacteria 和 Actinomycetota 的丰度明显更高。FL 和 PA 细菌群落的空间变化受扩散限制这一离散因素的影响最为显著。在环境参数中,发现总氮(TN)对 FL 和 PA 细菌群落的空间变化有显著影响(P
{"title":"Community composition and co-occurrence of free-living and particle-attached bacteria in the source region of the Ganges and Brahmaputra Rivers.","authors":"Namita Paudel Adhikari, Subash Adhikari, Komal Raj Rijal","doi":"10.1007/s10123-024-00607-6","DOIUrl":"https://doi.org/10.1007/s10123-024-00607-6","url":null,"abstract":"<p><p>Bacteria have two trophic lifestyles in aquatic ecosystems, i.e., free-living (FL) and particle-attached (PA), with different but essential ecological roles. However, relevant knowledge is still dearth in the upstream source region of the Himalayan Rivers. Thus, we emphasized a comparative study on community composition, co-occurrence, and geographic distribution of the FL and PA bacteria and the effect of environmental factors in the source region of the Ganges and Brahmaputra Rivers. PA bacteria relative to FL harbored a significantly higher local diversity, richness, and evenness. A significantly higher abundance of Betaproteobacteria, Verrucomicrobiota, and Planctomycetota in PA trophic lifestyle and Gammaproteobacteria and Actinomycetota in FL tropic lifestyle and indicator OTUs belonging to related taxa were observed. The spatial variation of the FL and PA bacterial communities was most significantly impacted by dispersal limitation as a discrete factor. Among the environmental parameters, the total nitrogen (TN) was found to be a significant (P < 0.001) driver of the variation in PA communities. Meanwhile, particulate organic carbon (POC) and TN considerably explained the variation of FL communities. A significant correlation (P < 0.001) of TN with dominant bacterial taxa (Pseudomonadota, Actinomycetota, and Verrucomicrobiota) and FL and PA indicator OTUs associated with these taxa further confirmed nitrogen as the limiting nutrient in the source region of the Ganges and Brahmaputra Rivers. The co-occurrence network topological characteristics showed that the PA network was more stable than the FL network, which was more complicated and unstable. Thus, it can be speculated that FL communities relative to PA are more vulnerable to shifting upon disturbances.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142465462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cured meat is a fermented meat product from the traditional Chinese culture made by natural fermentation. In this study, five bacteria strains were screened from cured meat using 16S rDNA technology, and a functional local starter was selected, which was applied to the production of cured meat to standardize the production of cured meat and improve the quality of cured meat. By studying the fermentation characteristics of strain these strains, this study found that the fermentation characteristics of L. mesenteroides and S. lactis are ideal. L. mesenteroides and S. lactis were used as starter cultures in fermented bacon. Then, this study compared the quality of fermented beef with Sichuan bacon, Hunan bacon, and Xinyang bacon. The results suggested that L. mesenteroides and S. lactis can improve the sensory and texture properties of the products and reduce the moisture content, water activity, pH value, and protein content of fermented beef products. More importantly, L. mesenteroides can significantly reduce the nitrite content (25.34%) and nitrosamine content (29.69%) in fermented beef, which provides an excellent guarantee for the safety of cured meat. In this study, a functional fermentation strain-L. mesenteroides could degrade the nitrite content of fermented meat products and improve their sensory and textural properties-was screened to provide some reference value for the later development of functional strains suitable for fermented meat products.
{"title":"Screening and application of functional autochthonous starter culture from cured meat, which can reduce nitrite content.","authors":"Qiuhui Zhang, Jialong Shen, Gaoge Meng, Han Wang, Chang Liu, Chaozhi Zhu, Gaiming Zhao","doi":"10.1007/s10123-024-00606-7","DOIUrl":"https://doi.org/10.1007/s10123-024-00606-7","url":null,"abstract":"<p><p>Cured meat is a fermented meat product from the traditional Chinese culture made by natural fermentation. In this study, five bacteria strains were screened from cured meat using 16S rDNA technology, and a functional local starter was selected, which was applied to the production of cured meat to standardize the production of cured meat and improve the quality of cured meat. By studying the fermentation characteristics of strain these strains, this study found that the fermentation characteristics of L. mesenteroides and S. lactis are ideal. L. mesenteroides and S. lactis were used as starter cultures in fermented bacon. Then, this study compared the quality of fermented beef with Sichuan bacon, Hunan bacon, and Xinyang bacon. The results suggested that L. mesenteroides and S. lactis can improve the sensory and texture properties of the products and reduce the moisture content, water activity, pH value, and protein content of fermented beef products. More importantly, L. mesenteroides can significantly reduce the nitrite content (25.34%) and nitrosamine content (29.69%) in fermented beef, which provides an excellent guarantee for the safety of cured meat. In this study, a functional fermentation strain-L. mesenteroides could degrade the nitrite content of fermented meat products and improve their sensory and textural properties-was screened to provide some reference value for the later development of functional strains suitable for fermented meat products.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142465465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The viability and functional activity of lactic acid bacteria (LAB), such as Lactobacillus acidophilus in a fermented milk product is important. One of the functional activities of the LAB in fermented milk is the ability of the LAB to positively impact the human health. This study aimed to determine the effect of frozen storage on the fermented milk, namely acidophilus milk, based on the nutritional milk quality, the viability of L. acidophilus, and its potential to lower cholesterol levels in Wistar rats. The parameters measured was including milk quality (mainly pH, protein content, lactic acid levels, and syneresis), L. acidophilus bacterial viability in frozen storage (for 1, 2, and 3 months), and some biological assays to evaluate the potential of milk in lowering blood cholesterol levels in Wistar rats. The result of this study suggests that acidophilus milk quality can be maintained in frozen storage for two months, and it had lactic acid levels of 1.07%, pH of 4.08, and protein levels of 3.33%. Giving acidophilus milk to Wistar rats for 15 days could reduce the cholesterol level continuously until 30 days of treatment. Therefore, this study proves that acidophilus milk quality can be maintained very well in frozen storage, and its functional properties to lower the cholesterol level of Wistar rats can be achieved after two weeks of consumption.
{"title":"Viability of Lactobacillus acidophilus in acidophilus milk during frozen storage and its potential to lower cholesterol: an In vivo study.","authors":"Yurliasni Yurliasni, Elmy Mariana, Yusdar Zakaria, Zuraida Hanum","doi":"10.1007/s10123-024-00605-8","DOIUrl":"https://doi.org/10.1007/s10123-024-00605-8","url":null,"abstract":"<p><p>The viability and functional activity of lactic acid bacteria (LAB), such as Lactobacillus acidophilus in a fermented milk product is important. One of the functional activities of the LAB in fermented milk is the ability of the LAB to positively impact the human health. This study aimed to determine the effect of frozen storage on the fermented milk, namely acidophilus milk, based on the nutritional milk quality, the viability of L. acidophilus, and its potential to lower cholesterol levels in Wistar rats. The parameters measured was including milk quality (mainly pH, protein content, lactic acid levels, and syneresis), L. acidophilus bacterial viability in frozen storage (for 1, 2, and 3 months), and some biological assays to evaluate the potential of milk in lowering blood cholesterol levels in Wistar rats. The result of this study suggests that acidophilus milk quality can be maintained in frozen storage for two months, and it had lactic acid levels of 1.07%, pH of 4.08, and protein levels of 3.33%. Giving acidophilus milk to Wistar rats for 15 days could reduce the cholesterol level continuously until 30 days of treatment. Therefore, this study proves that acidophilus milk quality can be maintained very well in frozen storage, and its functional properties to lower the cholesterol level of Wistar rats can be achieved after two weeks of consumption.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142465466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}