Pub Date : 2026-03-24DOI: 10.1007/s10123-025-00767-z
Haider Ammar Alubaidy, Ali Hamid Al-Bdeery, Zena Abd-Alkhadim Owda, Haider A Alghanmi, Suresh Ghotekar, Majid S Jabir
{"title":"In Silico and In-vitro assessment of β carotene extracted from Westella botryoides for fighting multidrug resistant bacterial strains.","authors":"Haider Ammar Alubaidy, Ali Hamid Al-Bdeery, Zena Abd-Alkhadim Owda, Haider A Alghanmi, Suresh Ghotekar, Majid S Jabir","doi":"10.1007/s10123-025-00767-z","DOIUrl":"https://doi.org/10.1007/s10123-025-00767-z","url":null,"abstract":"","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147503806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-19DOI: 10.1007/s10123-026-00800-9
Gaelle Chaaya, Marina Daccache, Joseph Yaghi, Nicolas Louka, Richard G Maroun, Jean Claude Assaf, André El Khoury, Roger Lteif
{"title":"Comparative fermentation performance of biofilm and planktonic Saccharomyces cerevisiae under standard and high-carbon media.","authors":"Gaelle Chaaya, Marina Daccache, Joseph Yaghi, Nicolas Louka, Richard G Maroun, Jean Claude Assaf, André El Khoury, Roger Lteif","doi":"10.1007/s10123-026-00800-9","DOIUrl":"https://doi.org/10.1007/s10123-026-00800-9","url":null,"abstract":"","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147486123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
<p><strong>Introduction: </strong>Fungal endophytes share a symbiotic relationship with the host plants. Endophytes from medicinal plants produce metabolites similar to plants as well as some new metabolites, which serve as a promising medicinal source with, significant potential in the field of biomedicine. Epigenetic modifiers, such as DNA methyltransferase and histone deacetylase inhibitors, activate cryptic biosynthesis gene clusters, resulting in a significant increase in cryptic metabolite production. This study elucidated the alteration in the metabolite profiles of two endophytes isolated from the medicinal plant Embelia ribes after treatment with two epigenetic modulators.</p><p><strong>Materials and methods: </strong>This study assessed the effect of epigenetic modifiers-Azacitidine (AZ) and Sodium butyrate (SB)-on the metabolite profiles of Phomopsis azadirachtae and Diaporthe phaseolorum. Different concentrations of AZ and SB (1, 10, 50, 100, and 500 mM) were employed to assess their impact on the fungal endophyte cultures. Metabolome analysis was performed to observe the alteration of metabolites.</p><p><strong>Results: </strong>LC-MS analysis revealed 47 targeted metabolites in the AZ-treated P. azadirachtae culture. Treatment with AZ significantly affected the production of metabolites compared with the control. AZ treatment also altered the production of nine silent metabolites; namely dicerandrol B, phomosine A, epiepoxydon, taxol, cladosporine, phomonaphthalenone A, phomophyllin A, 3-indolepropionic acid (3-IPA) and ergosterol in P. azadirachtae culture. Two metabolites enhanced their production compared to the control. A total of 47 metabolites were identified in P. azadirachtae culture treated with SB, which also altered 11 silent metabolites and enhanced production of six metabolites; cytosporone B, phomophyllin A, phomosine A, phomosin B, laiolactol A, and ergosterol P by logarithmic analysis. Similarly, 41 metabolites were identified in D. phaseolorum culture treated with various concentrations of AZ. In D. phaseolorum culture treated with AZ, an epigenetic modification activated 11 silent metabolites-Cytochalasin N, bostrycoidin, phomonaphthalenone, phomopsterone, dicerandrol A, pinselin, indole-3-acetic acid, betulinic acid, phomophyllin A, dalienxanthone B and phomopoxide A. Two metabolites, phomosine A and zeatin riboside, were enhanced in majority of the AZ treatments compared to control by logarithmic analysis. SB treatment significantly modulated the metabolite profile of D. phaseolorum, with LC-MS analysis detecting 46 targeted compounds across different concentrations. The treatment activated 11 previously silent bioactive metabolites, including Ganodermaside D, lithocarpinol A, dalienxanthone B, cladospirone, dicerandrol B, libertellenone, phomonaphthalenone A, phomopoxide A, phomopsichin B, phomopsterone B, and cladospirone. Three metabolites, pinselin, dicerandrol A, and phmosine A was significantly enhanced in
{"title":"Effect of epigenetic modulation on metabolites from endophytes isolated from Embelia ribes.","authors":"Ajinkya Terkar, Aditya Raut, Jayant Kulkarni, Vitthal T Barvkar, Mahesh Borde","doi":"10.1007/s10123-026-00796-2","DOIUrl":"https://doi.org/10.1007/s10123-026-00796-2","url":null,"abstract":"<p><strong>Introduction: </strong>Fungal endophytes share a symbiotic relationship with the host plants. Endophytes from medicinal plants produce metabolites similar to plants as well as some new metabolites, which serve as a promising medicinal source with, significant potential in the field of biomedicine. Epigenetic modifiers, such as DNA methyltransferase and histone deacetylase inhibitors, activate cryptic biosynthesis gene clusters, resulting in a significant increase in cryptic metabolite production. This study elucidated the alteration in the metabolite profiles of two endophytes isolated from the medicinal plant Embelia ribes after treatment with two epigenetic modulators.</p><p><strong>Materials and methods: </strong>This study assessed the effect of epigenetic modifiers-Azacitidine (AZ) and Sodium butyrate (SB)-on the metabolite profiles of Phomopsis azadirachtae and Diaporthe phaseolorum. Different concentrations of AZ and SB (1, 10, 50, 100, and 500 mM) were employed to assess their impact on the fungal endophyte cultures. Metabolome analysis was performed to observe the alteration of metabolites.</p><p><strong>Results: </strong>LC-MS analysis revealed 47 targeted metabolites in the AZ-treated P. azadirachtae culture. Treatment with AZ significantly affected the production of metabolites compared with the control. AZ treatment also altered the production of nine silent metabolites; namely dicerandrol B, phomosine A, epiepoxydon, taxol, cladosporine, phomonaphthalenone A, phomophyllin A, 3-indolepropionic acid (3-IPA) and ergosterol in P. azadirachtae culture. Two metabolites enhanced their production compared to the control. A total of 47 metabolites were identified in P. azadirachtae culture treated with SB, which also altered 11 silent metabolites and enhanced production of six metabolites; cytosporone B, phomophyllin A, phomosine A, phomosin B, laiolactol A, and ergosterol P by logarithmic analysis. Similarly, 41 metabolites were identified in D. phaseolorum culture treated with various concentrations of AZ. In D. phaseolorum culture treated with AZ, an epigenetic modification activated 11 silent metabolites-Cytochalasin N, bostrycoidin, phomonaphthalenone, phomopsterone, dicerandrol A, pinselin, indole-3-acetic acid, betulinic acid, phomophyllin A, dalienxanthone B and phomopoxide A. Two metabolites, phomosine A and zeatin riboside, were enhanced in majority of the AZ treatments compared to control by logarithmic analysis. SB treatment significantly modulated the metabolite profile of D. phaseolorum, with LC-MS analysis detecting 46 targeted compounds across different concentrations. The treatment activated 11 previously silent bioactive metabolites, including Ganodermaside D, lithocarpinol A, dalienxanthone B, cladospirone, dicerandrol B, libertellenone, phomonaphthalenone A, phomopoxide A, phomopsichin B, phomopsterone B, and cladospirone. Three metabolites, pinselin, dicerandrol A, and phmosine A was significantly enhanced in ","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147443540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-13DOI: 10.1007/s10123-026-00788-2
Aman Ullah, Talmeez Ur Rehman, Shahzar Khan, Qurban Ali, Mahwish Ali, Abdul Haleem, Sajid Iqbal, Mohammed Raashid, Abdul Haq, Safia Ahmed, Abebe Bogale, Fatmah M Alqahtani, Omar A Almohammed
{"title":"Extract of endophytic fungus Cepalotheca foveolata (N11) inhibits lytic bacteriophage: a study on activity, cytotoxicity, and chemical profiling.","authors":"Aman Ullah, Talmeez Ur Rehman, Shahzar Khan, Qurban Ali, Mahwish Ali, Abdul Haleem, Sajid Iqbal, Mohammed Raashid, Abdul Haq, Safia Ahmed, Abebe Bogale, Fatmah M Alqahtani, Omar A Almohammed","doi":"10.1007/s10123-026-00788-2","DOIUrl":"https://doi.org/10.1007/s10123-026-00788-2","url":null,"abstract":"","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147443471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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