Effects of Lactiplantibacillus plantarum LM1215 on Candida albicans and Gardnerella vaginalis.

IF 2.6 4区医学 Q1 MEDICINE, GENERAL & INTERNAL Yonsei Medical Journal Pub Date : 2024-12-01 DOI:10.3349/ymj.2023.0490

Won-Young Bae, Young Jin Lee, Subin Jo, So Lim Shin, Tae-Rahk Kim, Minn Sohn, Hyun-Joo Seol

{"title":"Effects of Lactiplantibacillus plantarum LM1215 on Candida albicans and Gardnerella vaginalis.","authors":"Won-Young Bae, Young Jin Lee, Subin Jo, So Lim Shin, Tae-Rahk Kim, Minn Sohn, Hyun-Joo Seol","doi":"10.3349/ymj.2023.0490","DOIUrl":null,"url":null,"abstract":"Purpose: The aim of this study was to identify novel vaginal probiotics with the potential to prevent vulvovaginal candidiasis (VVC) and bacterial vaginosis (BV).Materials and methods: Eighteen strains of Lactiplantibacillus plantarum were isolated from healthy Korean women, and their antimicrobial effects against Candida albicans and Gardnerella vaginalis were assessed. Three strains (L. plantarum LM1203, LM1209, and LM1215) were selected for further investigation, focusing on their growth inhibition, biofilm regulation, and cellular mechanisms against these vaginal pathogens. Additionally, electron microscopy revealed damage to G. vaginalis induced by L. plantarum LM1215, and genomic analysis was conducted on this strain.Results: L. plantarum LM1203, LM1209, and LM1215 showed approximately 1 and 2 Log CFU/mL growth reduction in C. albicans and G. vaginalis, respectively. These L. plantarum strains effectively inhibited biofilm formation and eliminated the mature biofilms formed by C. albicans. Furthermore, L. plantarum LM1215 decreased tricarboxylic acid cycle activity by 51.75 (p<0.001) and respiratory metabolic activity by 52.88% (p<0.001) in G. vaginalis. L. plantarum induced cellular membrane damage, inhibition of protein synthesis, and cell wall collapse in G. vaginalis. Genomic analysis confirmed L. plantarum LM1215 as a safe strain for vaginal probiotics.Conclusion: The L. plantarum LM1215 is considered a safe probiotic agent suitable for the prevention of VVC and BV.","PeriodicalId":23765,"journal":{"name":"Yonsei Medical Journal","volume":"65 12","pages":"727-740"},"PeriodicalIF":2.6000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11605040/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Yonsei Medical Journal","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3349/ymj.2023.0490","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose: The aim of this study was to identify novel vaginal probiotics with the potential to prevent vulvovaginal candidiasis (VVC) and bacterial vaginosis (BV).

Materials and methods: Eighteen strains of Lactiplantibacillus plantarum were isolated from healthy Korean women, and their antimicrobial effects against Candida albicans and Gardnerella vaginalis were assessed. Three strains (L. plantarum LM1203, LM1209, and LM1215) were selected for further investigation, focusing on their growth inhibition, biofilm regulation, and cellular mechanisms against these vaginal pathogens. Additionally, electron microscopy revealed damage to G. vaginalis induced by L. plantarum LM1215, and genomic analysis was conducted on this strain.

Results: L. plantarum LM1203, LM1209, and LM1215 showed approximately 1 and 2 Log CFU/mL growth reduction in C. albicans and G. vaginalis, respectively. These L. plantarum strains effectively inhibited biofilm formation and eliminated the mature biofilms formed by C. albicans. Furthermore, L. plantarum LM1215 decreased tricarboxylic acid cycle activity by 51.75 (p<0.001) and respiratory metabolic activity by 52.88% (p<0.001) in G. vaginalis. L. plantarum induced cellular membrane damage, inhibition of protein synthesis, and cell wall collapse in G. vaginalis. Genomic analysis confirmed L. plantarum LM1215 as a safe strain for vaginal probiotics.

Conclusion: The L. plantarum LM1215 is considered a safe probiotic agent suitable for the prevention of VVC and BV.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

植物乳杆菌LM1215对白色念珠菌和阴道加德纳菌的影响。

目的：本研究的目的是鉴定具有预防外阴阴道念珠菌病（VVC）和细菌性阴道病（BV）潜力的新型阴道益生菌。材料与方法：从韩国健康女性中分离到18株植物乳杆菌，观察其对白色念珠菌和阴道加德纳菌的抑菌效果。选择3株植物乳杆菌LM1203、LM1209和LM1215进行进一步研究，重点研究它们对阴道病原菌的生长抑制、生物膜调控和细胞机制。此外，电镜观察发现植物乳杆菌LM1215对阴道毛囊的损伤，并对该菌株进行了基因组分析。结果：植物乳杆菌LM1203、LM1209和LM1215对白色念珠菌和阴道念珠菌的生长分别减少约1和2 Log CFU/mL。这些植物乳杆菌菌株有效地抑制了白念珠菌形成的生物膜，并消除了白念珠菌形成的成熟生物膜。L. plantarum LM1215使三羧酸循环活性降低51.75 （ppG）。鞘突。植物乳杆菌诱导阴道菌细胞膜损伤、蛋白合成抑制和细胞壁塌陷。基因组分析证实植物乳杆菌LM1215是一种安全的阴道益生菌菌株。结论：植物乳杆菌LM1215是一种安全的益生菌制剂，适用于预防VVC和BV。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Yonsei Medical Journal 医学-医学：内科

CiteScore

4.50

自引率

0.00%

发文量

167

审稿时长

3 months

期刊介绍： The goal of the Yonsei Medical Journal (YMJ) is to publish high quality manuscripts dedicated to clinical or basic research. Any authors affiliated with an accredited biomedical institution may submit manuscripts of original articles, review articles, case reports, brief communications, and letters to the Editor.