Thymus-forming potential of the second pharyngeal pouch and its regulation by local mesenchyme in avian embryos.

IF 7.5 1区生物学 Q1 CELL BIOLOGY Cell reports Pub Date : 2024-12-24 Epub Date: 2024-11-29 DOI:10.1016/j.celrep.2024.114998

Isabel Alcobia, Margarida Gama-Carvalho, Leonor Magalhães, Vitor Proa, Sara Ferreira, Domingos Henrique, Hélia Neves

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Abstract

The thymus derives from the endoderm of pharyngeal pouches (PPs). The number and location of PPs with thymus-forming potential differ among jawed vertebrates, and ectopic thymus locations in mice and humans suggest a broader thymus-forming potential in PP endoderm than previously ascribed. We used the quail-chick chimera system to test if non-canonical pouches could form a thymus and examined the role of pharyngeal arch (PA) mesenchyme in this process. After testing several tissue associations, we identified thymus-forming potential in both non-canonical second PP and canonical third/fourth PP endoderm. We found the 3/4PA and the ventral region of 2PA mesenchyme to be capable of positively regulating this potential, while the dorsal region of 2PA exerts an inhibitory effect. Transcriptomic analysis revealed a shared genetic program associated with thymic potential in PP endoderm and uncovered distinct signaling pathways mediating cellular interactions between PP endoderm and PA mesenchyme, which modulate this thymic potential.

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来源期刊

Cell reports CELL BIOLOGY-

CiteScore

13.80

自引率

1.10%

发文量

1305

审稿时长

77 days

期刊介绍： Cell Reports publishes high-quality research across the life sciences and focuses on new biological insight as its primary criterion for publication. The journal offers three primary article types: Reports, which are shorter single-point articles, research articles, which are longer and provide deeper mechanistic insights, and resources, which highlight significant technical advances or major informational datasets that contribute to biological advances. Reviews covering recent literature in emerging and active fields are also accepted. The Cell Reports Portfolio includes gold open-access journals that cover life, medical, and physical sciences, and its mission is to make cutting-edge research and methodologies available to a wide readership. The journal's professional in-house editors work closely with authors, reviewers, and the scientific advisory board, which consists of current and future leaders in their respective fields. The advisory board guides the scope, content, and quality of the journal, but editorial decisions are independently made by the in-house scientific editors of Cell Reports.