Joint action of six-component mixtures based on concentration response curves morphological parameter in acute and long-term toxicity assay

Abstract

Previous studies found that the multi-component mixtures with hormesis concentration-response curves (CRCs) were divided into three types according to the combined toxicity analysis of the segment-based method and σ²_(k∙ECx) (the variance of k∙EC_x). In this study, the acute and long-term toxicity of six pollutants and 12 six-component mixtures were assessed using microplate toxicity analyses (MTA). The functional relationship between σ²_(k·ECx) and effect ratio (ER_x) was determined by means of the independent action (IA) and the ER model to systematically investigate the correlation between mixture types in acute and long-term toxicity. The results indicated that across the entire concentration range, the mixture type of acute toxicity was consistent with short time exposure (0.25 h) measured in the long-term toxicity experiment. In the inhibition effect range, the types of mixtures of acute toxicity remained consistent with the chronic toxicity (exposure for 24 h) in 11 of the 12 mixtures. This study clarified the changes in the joint action of multi-component mixtures on Aliivibrio fischeri in terms of acute and long-term toxicity. The chronic toxicity of the mixtures can be predicted from the acute toxicity results, which provides a theoretical basis for the biological toxicity evaluation of multi-component mixtures.