Abundance of fecal indicator bacteria and diversity of Escherichia coli associated with poultry farms and pasture land cover in streams of northwestern South Carolina

Abstract

Livestock can contribute fecal indicator bacteria (FIB) to waterbodies. However, few studies have examined the relationship between watershed land use or land cover involving livestock production and the genetic diversity of Escherichia coli in freshwater ecosystems. Our major goal was to determine if FIB abundance and E. coli phylogenetic group distributions in headwater streams are related to livestock production in rural watersheds in South Carolina. In both 2017 and 2018, grab samples were collected from streams at summer baseflow. In 2017, we collected samples from watersheds with or without poultry rearing facilities (PRFs). In 2018, we collected samples from streams draining watersheds with mixed forest and pasture cover and from streams in mostly forested watersheds. In both summers, we measured concentrations of total coliforms, E. coli, and Enterococcus. We also categorized E. coli isolates into one of four phylogenetic groups (A, B1, B2, D). Streams with PRFs in their watersheds had significantly higher concentrations of Enterococcus but not total coliforms or E. coli than streams in watersheds without PRFs. Also, B2 isolates were less frequent and B1 isolates were more frequent in watersheds with PRFs than in those without. Streams draining mixed forest/pasture watersheds had significantly higher concentrations of total coliforms and E. coli but not Enterococcus, as well as higher frequencies of B1 isolates, than streams in mostly forested watersheds. Overall, the most frequent E. coli phylogenetic groups in watersheds with animal production appeared consistent with the phylogenetic groups that are especially abundant in poultry or mammalian livestock feces.