Microplastics-exposure experience aggravates the accumulation of diarrhetic shellfish toxins (DSTs) in thick-shell mussel Mytilus coruscus through impairing detoxification processes

Abstract

Possessing sessile filter-feeding lifestyle, bivalves are more susceptible to contamination by benthic phycotoxins such as the diarrhetic shellfish toxins (DSTs). Due to the prevalence of microplastics (MPs) in aquatic environments, bivalve that experienced MP-exposure are potentially at higher risk from exposure to DSTs-producing microalgae, however, little is known about the impacts of past MP-exposure experience on the accumulation of DSTs. In this study, taking polystyrene (PS) MPs and DSTs-producing Prorocentrum lima as representatives, the impacts of MP-exposure on DSTs accumulation were evaluated in the thick-shell mussel Mytilus coruscus. Our results demonstrated that mussels with MP-exposure experience accumulated markedly higher levels of DSTs in their digestive glands, which may result from a significant impairment of detoxification. In addition, although might exert their effects through different mechanisms, both MP- and/or P. lima-exposure aggravated the level of oxidative stress and led to significant histological lesion of the digestive glands, with the highest stress and lesion observed in mussels that exposed to P. lima after a 21-day MP-exposure. Collectively, our results indicate the risk of DSTs-contamination of bivalves could be markedly aggravated by the ubiquitous presence of MPs, which may pose a severe threat to human consumers and warrants upmost attention.