Long Noncoding RNA EMX2-AS Facilitates Osteoblast Differentiation and Bone Formation by Inhibiting EMX2 Protein Translation and Activating Wnt/β-Catenin Pathway.

IF 3.8 3区 医学 Q2 CELL & TISSUE ENGINEERING Stem Cells International Pub Date : 2024-11-26 eCollection Date: 2024-01-01 DOI:10.1155/sci/4397807
Linyuan Fan, Li Zhang, Xin Zhang, Wei Wei, Zhaohui Liu
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Abstract

Long noncoding RNAs (lncRNAs), as a potentially new and crucial element of biological regulation, have gained widespread attention in recent years. Our previous work identified lncRNA empty spiracles homeobox 2 antisence (EMX2-AS) was significantly increased during the osteoblast differentiation of mesenchymal stem cells (MSCs). Overexpression of lncRNA EMX2-AS promoted osteogenesis in vitro and enhanced heterotopic bone formation in vivo, whereas lncRNA EMX2-AS knockdown had the opposite effect. EMX2 could negatively regulate the osteoblast differentiation of MSCs. lncRNA EMX2-AS was 80% expressed in the cytoplasm during osteoblast differentiation in MSCs. Mechanistic analysis revealed that lncRNA EMX2-AS acts as a positive regulator of osteogenic differentiation through interaction with EMX2 and suppression of its expression at the translational level and Wnt/β-catenin pathway is involved in lncRNA EMX2-AS/EMX2 regulated osteogenic differentiation. Our findings not only provide new targets for the treatment of diseases related to osteoblast differentiation disruption but also enrich the understanding of the regulation mechanisms of lncRNA during stem cell differentiation.

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长链非编码RNA EMX2- as通过抑制EMX2蛋白翻译和激活Wnt/β-Catenin通路促进成骨细胞分化和骨形成。
长链非编码rna (Long noncoding rna, lncRNAs)作为一种潜在的重要生物调控元件,近年来受到了广泛关注。我们之前的研究发现,在间充质干细胞(MSCs)成骨分化过程中,lncRNA空气门同源盒2反义性(EMX2-AS)显著增加。lncRNA EMX2-AS过表达在体外促进成骨,在体内增强异位骨形成,而lncRNA EMX2-AS敲低则具有相反的效果。EMX2负向调控MSCs成骨细胞分化。在MSCs成骨细胞分化过程中,lncRNA EMX2-AS在细胞质中有80%的表达。机制分析表明,lncRNA EMX2- as通过与EMX2相互作用并在翻译水平上抑制其表达,起到正向调控成骨分化的作用,而Wnt/β-catenin通路参与了lncRNA EMX2- as /EMX2调控成骨分化的过程。我们的发现不仅为成骨细胞分化中断相关疾病的治疗提供了新的靶点,而且丰富了对lncRNA在干细胞分化过程中的调控机制的认识。
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来源期刊
Stem Cells International
Stem Cells International CELL & TISSUE ENGINEERING-
CiteScore
8.10
自引率
2.30%
发文量
188
审稿时长
18 weeks
期刊介绍: Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and clinical research, including animal models and clinical trials. Topics covered include, but are not limited to: embryonic stem cells; induced pluripotent stem cells; tissue-specific stem cells; stem cell differentiation; genetics and epigenetics; cancer stem cells; stem cell technologies; ethical, legal, and social issues.
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