Facile and versatile PDMS-glass capillary double emulsion formation device coupled with rapid purification toward microfluidic giant liposome generation.

IF 7.3 1区 工程技术 Q1 INSTRUMENTS & INSTRUMENTATION Microsystems & Nanoengineering Pub Date : 2024-12-05 DOI:10.1038/s41378-024-00815-0
Mostafa Bakouei, Ali Kalantarifard, Indraja Sundara Raju, Tatiana Avsievich, Lauri Rannaste, Marjut Kreivi, Caglar Elbuken
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Abstract

The exceptional ability of liposomes to mimic a cellular lipid membrane makes them invaluable tools in biomembrane studies and bottom-up synthetic biology. Microfluidics provides a promising toolkit for creating giant liposomes in a controlled manner. Nevertheless, challenges associated with the microfluidic formation of double emulsions, as precursors to giant liposomes, limit the full exploration of this potential. In this study, we propose a PDMS-glass capillary hybrid device as a facile and versatile tool for the formation of double emulsions which not only eliminates the need for selective surface treatment, a well-known problem with PDMS formation chips, but also provides fabrication simplicity and reusability compared to the glass-capillary formation chips. These advantages make the presented device a versatile tool for forming double emulsions with varying sizes (spanning two orders of magnitude in diameter), shell thickness, number of compartments, and choice of solvents. We achieved robust thin shell double emulsion formation by operating the hybrid chip in double dripping mode without performing hydrophilic/phobic treatment a priori. In addition, as an alternative to the conventional, time-consuming density-based separation method, a tandem separation chip is developed to deliver double emulsions free of any oil droplet contamination in a continuous and rapid manner without any need for operator handling. The applicability of the device was demonstrated by forming giant liposomes using the solvent extraction method. This easy-to-replicate, flexible, and reliable microfluidic platform for the formation and separation of double emulsion templates paves the way for the high-throughput microfluidic generation of giant liposomes and synthetic cells, opening exciting avenues for biomimetic research. The presented giant liposome assembly line features a novel treatment-free hybrid chip for double emulsion formation coupled with a high throughput separation chip for sample purification.

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简易多功能的pdms -玻璃毛细管双乳形成装置,结合微流控巨脂质体生成的快速净化。
脂质体模拟细胞脂膜的特殊能力使其成为生物膜研究和自下而上合成生物学的宝贵工具。微流体技术为以可控的方式制造巨脂质体提供了一个很有前途的工具。然而,双乳的微流体形成的挑战,作为巨大脂质体的前体,限制了这一潜力的充分探索。在这项研究中,我们提出了一种PDMS-玻璃毛细管混合装置,作为一种简单而通用的工具,用于形成双乳液,它不仅消除了PDMS形成芯片的一个众所周知的问题,即需要选择性表面处理,而且与玻璃毛细管形成芯片相比,它还提供了制造简单和可重用性。这些优点使该装置成为形成具有不同尺寸(直径跨越两个数量级)、壳层厚度、室数和溶剂选择的双乳液的通用工具。我们通过在双滴模式下操作混合芯片,而无需进行先验的亲疏水处理,实现了坚固的薄壳双乳液形成。此外,作为传统的耗时的基于密度的分离方法的替代方案,开发了一种串联分离芯片,可以连续快速地提供没有任何油滴污染的双乳,而无需操作人员处理。通过溶剂萃取法形成巨脂质体,证明了该装置的适用性。这种易于复制、灵活和可靠的微流控平台用于双乳液模板的形成和分离,为巨脂质体和合成细胞的高通量微流控生成铺平了道路,为仿生研究开辟了令人兴奋的途径。所提出的巨型脂质体装配线具有一种新型的用于双乳形成的免处理混合芯片以及用于样品纯化的高通量分离芯片。
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来源期刊
Microsystems & Nanoengineering
Microsystems & Nanoengineering Materials Science-Materials Science (miscellaneous)
CiteScore
12.00
自引率
3.80%
发文量
123
审稿时长
20 weeks
期刊介绍: Microsystems & Nanoengineering is a comprehensive online journal that focuses on the field of Micro and Nano Electro Mechanical Systems (MEMS and NEMS). It provides a platform for researchers to share their original research findings and review articles in this area. The journal covers a wide range of topics, from fundamental research to practical applications. Published by Springer Nature, in collaboration with the Aerospace Information Research Institute, Chinese Academy of Sciences, and with the support of the State Key Laboratory of Transducer Technology, it is an esteemed publication in the field. As an open access journal, it offers free access to its content, allowing readers from around the world to benefit from the latest developments in MEMS and NEMS.
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