Platelet-rich fibrin associated to bovine bone induces bone regeneration in model of critical-sized calvaria defect of rats submitted to Zoledronic Acid therapy

IF 2 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Journal of Stomatology Oral and Maxillofacial Surgery Pub Date : 2025-09-01 Epub Date: 2024-12-02 DOI:10.1016/j.jormas.2024.102175

Bárbara Gressy Duarte Souza Carneiro , Thays Allane Cordeiro Maia , Vanessa Costa , Simone Peixe Frieddrichsdorf , Delane Viana Gondim , Karuza Maria Alves Pereira , Mirna Marques Bezerra , Paula Goes

{"title":"Platelet-rich fibrin associated to bovine bone induces bone regeneration in model of critical-sized calvaria defect of rats submitted to Zoledronic Acid therapy","authors":"Bárbara Gressy Duarte Souza Carneiro , Thays Allane Cordeiro Maia , Vanessa Costa , Simone Peixe Frieddrichsdorf , Delane Viana Gondim , Karuza Maria Alves Pereira , Mirna Marques Bezerra , Paula Goes","doi":"10.1016/j.jormas.2024.102175","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><div><span>Reconstruction of bone defects<span> prior to implant installation is a challenge, especially when the patient uses bisphosphonates. Given this difficulty, many studies investigate biomaterials that can improve the </span></span>bone regeneration<span><span><span> process. In this context, this study aimed to investigate the effect of platelet-rich fibrin (PRF) and Bio-Oss (BO) on bone regeneration of </span>rats submitted to critical-sized </span>calvaria<span> defects and treated with ZA.</span></span></div></div><div><h3>Methods</h3><div><span><span>Thirty Wistar rats received a </span>single dose<span> of ZA (120 μg/kg) and after 7 days, were submitted to an 8 mm calvaria defect. The animals were divided into 5 groups (</span></span><em>n</em> = 6): ZA, BO, PRF or BO+PRF; animals from control group did not receive ZA. All animals were euthanized 12 weeks after surgical procedure and calvaria collected to histological, histomorphometric and micro-CT analyses.</div></div><div><h3>Results</h3><div><span><span><span>BO+PRF increased the number of osteoblasts (33 %) and </span>osteoclasts (58 %), as well as blood vessels (70 %) and </span>Type I collagen (52 %) (</span><em>p</em> < 0.05) compared to ZA group.</div></div><div><h3>Conclusion</h3><div>In summary, the association of BO+PRF improved bone healing of large bone defect in rats receiving ZA and this may be an interesting approach for the treatment to be tested in patients under anti-resorptive therapy.</div></div>","PeriodicalId":55993,"journal":{"name":"Journal of Stomatology Oral and Maxillofacial Surgery","volume":"126 4","pages":"Article 102175"},"PeriodicalIF":2.0000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Stomatology Oral and Maxillofacial Surgery","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2468785524004646","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/12/2 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose

Reconstruction of bone defects prior to implant installation is a challenge, especially when the patient uses bisphosphonates. Given this difficulty, many studies investigate biomaterials that can improve the bone regeneration process. In this context, this study aimed to investigate the effect of platelet-rich fibrin (PRF) and Bio-Oss (BO) on bone regeneration of rats submitted to critical-sized calvaria defects and treated with ZA.

Methods

Thirty Wistar rats received a single dose of ZA (120 μg/kg) and after 7 days, were submitted to an 8 mm calvaria defect. The animals were divided into 5 groups (n = 6): ZA, BO, PRF or BO+PRF; animals from control group did not receive ZA. All animals were euthanized 12 weeks after surgical procedure and calvaria collected to histological, histomorphometric and micro-CT analyses.

Results

BO+PRF increased the number of osteoblasts (33 %) and osteoclasts (58 %), as well as blood vessels (70 %) and Type I collagen (52 %) (p < 0.05) compared to ZA group.

Conclusion

In summary, the association of BO+PRF improved bone healing of large bone defect in rats receiving ZA and this may be an interesting approach for the treatment to be tested in patients under anti-resorptive therapy.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

与牛骨相关的富血小板纤维蛋白在唑来膦酸治疗的大鼠临界大小颅骨缺损模型中诱导骨再生：PRF诱导骨愈合。

目的：骨缺损在植入前的重建是一个挑战，特别是当患者使用双磷酸盐时。鉴于这一困难，许多研究都在研究可以改善骨再生过程的生物材料。在此背景下，本研究旨在探讨富血小板纤维蛋白（PRF）和Bio-Oss （BO）对ZA治疗的大鼠临界尺寸颅骨缺损骨再生的影响。方法：30只Wistar大鼠给予单剂量ZA (120 μg/kg)， 7 d后造颅缺损8 mm。将动物分为5组（n=6）： ZA、BO、PRF或BO+PRF；对照组不给予ZA治疗。所有动物在手术后12周被安乐死，并收集颅骨进行组织学、组织形态学和显微ct分析。结果：BO+PRF增加了成骨细胞（33%）和破骨细胞（58%）的数量，增加了血管（70%）和I型胶原（52%）的数量。结论：综上所述，BO+PRF的关联促进了ZA大鼠大骨缺损的骨愈合，这可能是一种有趣的治疗方法，可以在抗吸收治疗的患者中进行试验。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊