Effect of COL11A1 on oral squamous cell carcinoma.

IF 2.2 3区医学 Q2 Dentistry Journal of Stomatology Oral and Maxillofacial Surgery Pub Date : 2024-12-03 DOI:10.1016/j.jormas.2024.102183

Yuan-Hang Liu, Dan Li, Hao-Lei Zhang, Bo-Hao Zhang, Wei-Jing Song, Tian-Ke Li

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Abstract

Background: Oral squamous cell carcinoma (OSCC) is the most common malignant tumor of the oral cavity, which is mainly a series of atypical hyperplasia of oral epithelial cells, and the overall prognosis remains poor.

Methods: GSE37991 and GSE38517 were downloaded from gene expression omnibus (GEO) to identify differentially expressed genes (DEGs). Functional enrichment analysis of DEGs was performed, weighted gene co-expression network analysis (WGCNA) was used. Protein-protein interaction (PPI) network was constructed. Core gene expression was visualized using a heatmap. Comparative toxicogenomics database (CTD) and miRNA analyses identified related diseases and regulatory miRNAs. Western blot (WB) was conducted to examine expression of COL11A1 and TGF-SMAD signaling components in OSCC samples.

Results: 5163 DEGs were identified. DEGs were enriched in metabolic processes and signaling pathways, including TGF-β/SMAD and PI3K-Akt. WGCNA identified 11 core modules. PPI network analysis revealed five core genes: COL11A1, AURKA, MELK, CCNA2, and BUB1. Heatmap analysis showed that COL11A1 is highly expressed in OSCC. CTD analysis indicated that COL11A1 is associated with OSCC. miRNA prediction identified potential regulatory factors. Western blot analysis demonstrated that COL11A1 is overexpressed in OSCC and is associated with TGF-SMAD signaling, inflammation, and cell cycle progression.

Conclusion: COL11A1 is highly expressed in OSCC and may serve as a target gene interacting with the TGF-SMAD signaling pathway.

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COL11A1对口腔鳞状细胞癌的影响。

背景：口腔鳞状细胞癌（Oral squamous cell carcinoma， OSCC）是口腔最常见的恶性肿瘤，主要为一系列口腔上皮细胞的不典型增生，总体预后较差。方法：从gene expression omnibus （GEO）下载GSE37991和GSE38517，鉴定差异表达基因（DEGs）。采用加权基因共表达网络分析（WGCNA），对deg进行功能富集分析。构建蛋白-蛋白相互作用（PPI）网络。使用热图可视化核心基因表达。比较毒物基因组数据库（CTD）和miRNA分析确定了相关疾病和调节miRNA。Western blot （WB）检测OSCC样品中COL11A1和TGF-SMAD信号组分的表达。结果：共鉴定出5163个deg。DEGs在代谢过程和信号通路中富集，包括TGF-β/SMAD和PI3K-Akt。WGCNA确定了11个核心模块。PPI网络分析显示了五个核心基因：COL11A1、AURKA、MELK、CCNA2和BUB1。热图分析显示COL11A1在OSCC中高表达。CTD分析显示COL11A1与OSCC相关。miRNA预测发现了潜在的调节因子。Western blot分析显示，COL11A1在OSCC中过表达，并与TGF-SMAD信号、炎症和细胞周期进展有关。结论：COL11A1在OSCC中高表达，可能是与TGF-SMAD信号通路相互作用的靶基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Stomatology Oral and Maxillofacial Surgery Dentistry-Oral Surgery

CiteScore

2.20

自引率

9.10%

发文量

305

期刊介绍： J Stomatol Oral Maxillofac Surg publishes research papers and techniques - (guest) editorials, original articles, reviews, technical notes, case reports, images, letters to the editor, guidelines - dedicated to enhancing surgical expertise in all fields relevant to oral and maxillofacial surgery: from plastic and reconstructive surgery of the face, oral surgery and medicine, … to dentofacial and maxillofacial orthopedics. Original articles include clinical or laboratory investigations and clinical or equipment reports. Reviews include narrative reviews, systematic reviews and meta-analyses. All manuscripts submitted to the journal are subjected to peer review by international experts, and must: Be written in excellent English, clear and easy to understand, precise and concise; Bring new, interesting, valid information - and improve clinical care or guide future research; Be solely the work of the author(s) stated; Not have been previously published elsewhere and not be under consideration by another journal; Be in accordance with the journal''s Guide for Authors'' instructions: manuscripts that fail to comply with these rules may be returned to the authors without being reviewed. Under no circumstances does the journal guarantee publication before the editorial board makes its final decision. The journal is indexed in the main international databases and is accessible worldwide through the ScienceDirect and ClinicalKey Platforms.