Voltammetric analysis of glycoproteins containing sialylated and neutral glycans at pyrolytic graphite electrode

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioelectrochemistry Pub Date : 2024-11-28 DOI:10.1016/j.bioelechem.2024.108851

Mojmír Trefulka, Hana Černocká, Tatiana Staroňová, Veronika Ostatná

引用次数: 0

Abstract

Recently, it was described that neutral glycans can be distinguished from those containing sialic acid at the mercury electrode after modification with osmium(VI) N,N,N',N'-tetramethylethylenediamine (Os(VI)tem). Our work shows the possibility of studying glycans and glycoproteins at pyrolytic graphite electrodes depending on the presence of sialic acid.

Short glycans, glycans released from glycoproteins, and glycoproteins themselves yielded similar voltammetric responses after their modification by Os(VI)tem. Os(VI)tem modified glycans and glycoproteins produced a couple of cathodic and anodic peaks. Changing peak heights and potentials of glycans and glycoproteins pointed out the presence of sialic acid. These findings could be utilized to improve glycoprotein sensing by chemical modification.

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含唾液化和中性聚糖的糖蛋白在热解石墨电极上的伏安分析。

最近有报道称，在汞电极上用锇（VI） N，N，N‘，N’-四亚甲基乙二胺（Os(VI)tem）修饰后，可以区分中性聚糖和含有唾液酸的聚糖。我们的工作表明，根据唾液酸的存在，在热解石墨电极上研究聚糖和糖蛋白的可能性。短聚糖、糖蛋白释放的聚糖和糖蛋白本身经Os(VI)tem修饰后产生相似的伏安反应。Os(VI)tem修饰的聚糖和糖蛋白产生一对阴极和阳极峰。多糖和糖蛋白峰高和电位的变化表明唾液酸的存在。这些发现可用于通过化学修饰改善糖蛋白传感。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.