ADAM17 promotes colorectal cancer migration and invasion by regulating the TGF-β/Smad signaling pathway

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY Tissue & cell Pub Date : 2025-04-01 Epub Date: 2024-12-01 DOI:10.1016/j.tice.2024.102648

Jiaming Li , Tingjin Zheng , Yingzhi Xu , Mengcha Tian , Liangpan Shi , Jintu Chen , Tian Li , Zhishan Zhang

{"title":"ADAM17 promotes colorectal cancer migration and invasion by regulating the TGF-β/Smad signaling pathway","authors":"Jiaming Li , Tingjin Zheng , Yingzhi Xu , Mengcha Tian , Liangpan Shi , Jintu Chen , Tian Li , Zhishan Zhang","doi":"10.1016/j.tice.2024.102648","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>Investigate ADAM17 expression in colorectal cancer (CRC) at molecular and cellular levels and its potential mechanism in promoting tumorigenesis by regulating CRC cell migration and invasion.</div></div><div><h3>Materials and Methods</h3><div>The study measured ADAM17 mRNA and protein levels in colorectal cancer cells and tissues using qPCR and immunohistochemical staining, and assessed the cells' proliferation, migration, and invasion abilities.</div></div><div><h3>Results</h3><div>ADAM17 expression was significantly higher in CRC tissues than in non-cancerous tissues and was linked to metastasis and poor prognosis in CRC patients. Silencing ADAM17 reduced cell migration and invasion. Mechanistically, knocking down ADAM17 decreased the expression of TGF-β/Smad pathway-related proteins, which inhibited proteins associated with migration and invasion, thus impairing these cellular processes.</div></div><div><h3>Conclusion</h3><div>ADAM17 likely promotes the migration and invasion of CRC cells by regulating the TGF-β/Smad signaling pathway. This study aids in understanding the molecular mechanisms of CRC metastasis and development, and supports the development of new therapeutic targets.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"Article 102648"},"PeriodicalIF":2.5000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tissue & cell","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0040816624003495","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/12/1 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Objective

Investigate ADAM17 expression in colorectal cancer (CRC) at molecular and cellular levels and its potential mechanism in promoting tumorigenesis by regulating CRC cell migration and invasion.

Materials and Methods

The study measured ADAM17 mRNA and protein levels in colorectal cancer cells and tissues using qPCR and immunohistochemical staining, and assessed the cells' proliferation, migration, and invasion abilities.

Results

ADAM17 expression was significantly higher in CRC tissues than in non-cancerous tissues and was linked to metastasis and poor prognosis in CRC patients. Silencing ADAM17 reduced cell migration and invasion. Mechanistically, knocking down ADAM17 decreased the expression of TGF-β/Smad pathway-related proteins, which inhibited proteins associated with migration and invasion, thus impairing these cellular processes.

Conclusion

ADAM17 likely promotes the migration and invasion of CRC cells by regulating the TGF-β/Smad signaling pathway. This study aids in understanding the molecular mechanisms of CRC metastasis and development, and supports the development of new therapeutic targets.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

ADAM17通过调节TGF-β/Smad信号通路促进结直肠癌的迁移和侵袭。

目的：探讨ADAM17在结直肠癌（CRC）中分子和细胞水平的表达及其通过调节结直肠癌细胞迁移和侵袭促进肿瘤发生的潜在机制。材料与方法：采用qPCR和免疫组化染色检测结直肠癌细胞和组织中ADAM17 mRNA和蛋白水平，并评估细胞的增殖、迁移和侵袭能力。结果：ADAM17在结直肠癌组织中的表达明显高于非癌组织，并与结直肠癌患者的转移和预后不良有关。沉默ADAM17可减少细胞迁移和侵袭。从机制上讲，敲除ADAM17会降低TGF-β/Smad通路相关蛋白的表达，从而抑制与迁移和侵袭相关的蛋白，从而损害这些细胞过程。结论：ADAM17可能通过调节TGF-β/Smad信号通路促进结直肠癌细胞的迁移和侵袭。该研究有助于了解结直肠癌转移和发展的分子机制，并为开发新的治疗靶点提供支持。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

文献相关原料

公司名称

产品信息

索莱宝

Cell separation medium

来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.