{"title":"Quality control of corn silk extract using IR spectroscopy along with statistical methods.","authors":"Aiko Sano, Yutaka Inoue, Yuji Higuchi, Ken-Ichi Akao, Ryuichiro Suzuki","doi":"10.1007/s44211-024-00699-0","DOIUrl":null,"url":null,"abstract":"<p><p>Aqueous extracts of corn silk exhibit glycation-inhibitory activity. Lignin is the active component of these extracts. As corn silk is highly nutritious and has medicinal value, it can be used as a functional food and cosmetics. However, to achieve this goal, it is necessary to evaluate its quality. As lignin, which could be used as a marker compound for quality control, is a macromolecule, HPLC cannot be employed for the quality control of the aqueous extracts of corn silk. We here develop a method to evaluate the anti-glycation activity of the aqueous extracts of corn silk using attenuated total reflectance (ATR)-Fourier transform infrared (FTIR) spectroscopy along with multivariate statistical analysis. The inhibitory activity was evaluated using two multivariate calibrations: principal component regression (PCR) and partial least square regression (PLSR). The spectral areas of the PCR model were 633.5-880.3, 1191.8-1359.6, 1423.1-1492.6, and 2572.6-2974.7 cm<sup>-1</sup>. Its coefficient of correlation (R<sup>2</sup> = 0.981) and root mean square error of cross validation (RMSECV = 2.356) were highly predictable. The spectral regions of 983.5-985.5 and 1021.1-1107.9 cm<sup>-1</sup> offered the best prediction models for the PLSR model. The R<sup>2</sup> value for the correlation between the actual values and the FTIR-predicted values was 0.994, while the corresponding RMSECV was 1.325%. Hence, FTIR spectroscopy along with multivariate calibration is a useful method for evaluating active corn silk aqueous extracts.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Sciences","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s44211-024-00699-0","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Aqueous extracts of corn silk exhibit glycation-inhibitory activity. Lignin is the active component of these extracts. As corn silk is highly nutritious and has medicinal value, it can be used as a functional food and cosmetics. However, to achieve this goal, it is necessary to evaluate its quality. As lignin, which could be used as a marker compound for quality control, is a macromolecule, HPLC cannot be employed for the quality control of the aqueous extracts of corn silk. We here develop a method to evaluate the anti-glycation activity of the aqueous extracts of corn silk using attenuated total reflectance (ATR)-Fourier transform infrared (FTIR) spectroscopy along with multivariate statistical analysis. The inhibitory activity was evaluated using two multivariate calibrations: principal component regression (PCR) and partial least square regression (PLSR). The spectral areas of the PCR model were 633.5-880.3, 1191.8-1359.6, 1423.1-1492.6, and 2572.6-2974.7 cm-1. Its coefficient of correlation (R2 = 0.981) and root mean square error of cross validation (RMSECV = 2.356) were highly predictable. The spectral regions of 983.5-985.5 and 1021.1-1107.9 cm-1 offered the best prediction models for the PLSR model. The R2 value for the correlation between the actual values and the FTIR-predicted values was 0.994, while the corresponding RMSECV was 1.325%. Hence, FTIR spectroscopy along with multivariate calibration is a useful method for evaluating active corn silk aqueous extracts.
期刊介绍:
Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods.
This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.