Genomic characterization of the NAC transcription factors in carnation and function analysis of DcNAC41 involved in thermotolerance.

IF 6.1 2区 生物学 Q1 PLANT SCIENCES Plant Physiology and Biochemistry Pub Date : 2024-12-06 DOI:10.1016/j.plaphy.2024.109390
Mei Zhao, Ziyi Liu, Pengcheng Xue, Xiaojing Zhang, Xueli Wan
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Abstract

As pivotal regulators unique to plants, NAC family extensively orchestrate various life processes ranging from seed germination through growth and development to responses to environmental stresses. This study unraveled 71 NAC TFs in the carnation (Dianthus caryophyllus L.) genome, designated as DcNAC1 to DcNAC71, encoding amino acid sequences ranging from 80 to 718 residues. Subcellular localization predictions revealed a predominance of nuclear localization among these DcNACs. Phylogenetic analysis classified DcNACs into 14 distinct subgroups, each exhibiting similar gene structures and motifs. Promoter analysis highlighted the abundance of cis-regulatory elements (CREs) associated with plant growth and development regulation, hormone signaling, light response, and diverse stress responses, with stress-responsive CREs being the most prevalent, with at least one stress-responsive CRE detected in all DcNAC promoters. To assess their functional roles, 12 DcNACs, were randomly selected from different subgroups for expression profiling under heat, ABA, cold, and salt stress conditions, revealing distinct expression patterns for specific stress types. Notably, DcNAC41, which exhibited marked up-regulation under heat stress, was isolated and subsequently transformed into Arabidopsis. In heat-stressed conditions, transgenic Arabidopsis overexpressing DcNAC41 exhibited significant improvements in growth performance, survival rates, enhanced photosynthetic capacity, and strengthened ROS scavenging abilities. This study offers valuable insights into the comprehensive response of carnation DcNACs towards heat stress, particularly underscoring the potential of DcNAC41 as a promising candidate for enhancing thermotolerance in plants.

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作为植物特有的关键调控因子,NAC 家族广泛协调着从种子萌发、生长发育到环境胁迫响应的各种生命过程。本研究揭示了康乃馨基因组中的 71 个 NAC TFs,命名为 DcNAC1 至 DcNAC71,编码的氨基酸序列从 80 个残基到 718 个残基不等。亚细胞定位预测显示,这些 DcNACs 主要进行核定位。系统发育分析将 DcNACs 分成 14 个不同的亚群,每个亚群都表现出相似的基因结构和基调。启动子分析强调了与植物生长和发育调控、激素信号转导、光反应和各种胁迫反应有关的顺式调控元件(CRE)的丰富性,其中胁迫反应 CRE 最为普遍,在所有 DcNAC 启动子中至少检测到一个胁迫反应 CRE。为了评估它们的功能作用,研究人员从不同亚群中随机选取了 12 个 DcNACs,在热、ABA、冷和盐胁迫条件下进行了表达谱分析,发现了特定胁迫类型下的不同表达模式。值得注意的是,DcNAC41 在热胁迫下表现出明显的上调,被分离出来并随后转化到拟南芥中。在热胁迫条件下,过表达 DcNAC41 的转基因拟南芥在生长性能、存活率、光合能力和清除 ROS 能力方面都有显著改善。这项研究为了解康乃馨 DcNACs 对热胁迫的综合响应提供了宝贵的见解,特别是强调了 DcNAC41 作为增强植物耐热性的候选基因的潜力。
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来源期刊
Plant Physiology and Biochemistry
Plant Physiology and Biochemistry 生物-植物科学
CiteScore
11.10
自引率
3.10%
发文量
410
审稿时长
33 days
期刊介绍: Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
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