Selective aptasensor of deoxynivalenol based on dual signal enhancement of thionine electrochemistry using silver nanoparticle-loaded label at gold nanoparticle-loaded electrodes.

IF 4.8 2区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioelectrochemistry Pub Date : 2024-12-09 DOI:10.1016/j.bioelechem.2024.108881
Xiaoying Cui, Ke Zhao, Zhengquan Qu, Xipeng Chao, Lingling Xie, Hanyu Chen, Baoshan He, Baozhong Zhang
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Abstract

In this work, an efficient sensing platform deoxynivalenol (DON) detection was constructed through monitoring the current change of a competitive mechanism triggered by DON, leading the signal label detached from the electrode surface by square-wave voltammetry using thionine (Thi) as a redox indicator. The complementary strand of aptamer (cDNA) and Thi were loaded onto Fe/Ni bimetallic metal-organic framework loaded with sliver nanoparticles (AgNPs@FeNi-MOF) to construct AgNPs@FeNi-MOF/cDNA/Thi signal probes. In the presence of DON, the aptamer sequence was more predisposed to form an aptamer-DON complex, resulting in the displacement of the cDNA. The signal probe was subsequently released, leading to a decrease in the signal intensity of Thi. Notably, AgNPs@FeNi-MOF has a larger electroactive specific surface area and is able to load more cDNA and thi, which can amplify the signal. Under the optimal experimental conditions, the developed sensor exhibits a good linear response in the range of 1 × 10-2 to 1 × 104 pg/mL, with a limit of detection (LOD) of 5.68 fg/mL and has good selectivity, reproducibility and stability.

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上海源叶 Aflatoxin B1 (AFB1)
上海源叶 Fumonisin B1 (FB1)
上海源叶 Deoxynivalenol (DON)
上海源叶 silver nitrate (AgNO3)
上海源叶 Zearalenone (ZEN)
上海源叶 bovine serum albumin (BSA)
麦克林 sodium hydroxide (NaOH)
阿拉丁 Sodium borohydride (NaBH4)
来源期刊
Bioelectrochemistry
Bioelectrochemistry 生物-电化学
CiteScore
9.10
自引率
6.00%
发文量
238
审稿时长
38 days
期刊介绍: An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.
期刊最新文献
Corrigendum to "Analysis of electromagnetic response of cells and lipid membranes using a model-free method" [Bioelectrochemistry 152 (2023) 108444]. Corrigendum to "Molecular monolayers on silicon as substrates for biosensors" [Bioelectrochem. 80(1) (2010) 17-25]. Highly sensitive electrochemical Osteoprotegerin (OPG) immunosensor for assessing fracture healing and evaluating drug efficacy. Selective aptasensor of deoxynivalenol based on dual signal enhancement of thionine electrochemistry using silver nanoparticle-loaded label at gold nanoparticle-loaded electrodes. Investigation of a self-powered biosensor using a brush-based triboelectric nanogenerator and an enzymatic reaction.
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