Protective effect of trehalose on sperm chromatin condensation failure and semen quality decline in BDE-209-exposed mice

IF 3.9 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY Food and Chemical Toxicology Pub Date : 2025-02-01 DOI:10.1016/j.fct.2024.115168

Xin Gao , Huan He , Qi Zheng , Siju Chen , Yu Wei , Taifa Zhang , Yi Wang , Bo Wang , Dake Huang , Shengquan Zhang , Sumei Zhang , Jinxia Zhai

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Abstract

BDE-209 exposure induced male reproductive toxicity with sperm quality decline. However, the role of autophagy in this was unclear. The purpose was to evaluate the protective effect and its potential mechanism of trehalose (Tre, autophagy inducer) on reproductive damage during spermiogenesis induced by BDE-209. We used 2% w/v Tre and 75 mg/kg/d BDE-209 cotreated mice for 42 days. GC-2 spd cells were cotreated with Tre, chloroquine (CQ, inhibition of autophagic flux), compound C (CC, AMPK inhibitor), and BDE-209. Tre intake significantly recovered decrease in sexual organ ratio and poor sperm quality in BDE-209-exposed mice. Supplementation with Tre rescued sperm head malformation by improving aberrant histone-protamine exchange in BDE-209-exposed mice. However, Tre intake couldn't restore the acrosome biogenesis. In addition, Tre supplementation improved testicular damage induced by BDE-209. BDE-209 blocked autophagic flux with increased P62 and LC3BⅡ/Ⅰ levels. Mechanistically, CQ treatment aggravated elevation of P62 and LC3BⅡ/Ⅰ levels induced by BDE-209, otherwise, CC and Tre treatments inhibited the rise in p-AMPK, p-ULK1, P62 and LC3BⅡ/Ⅰ levels induced by BDE-209. Tre supplementation improved reproductive injury in BDE-209-exposed mice by regulating autophagic flow via AMPK-ULK1 signaling pathways, which providing a new theoretical basis and possible therapeutic targets for male reproductive toxicity.

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海藻糖对bde -209暴露小鼠精子染色质凝结失败和精液质量下降的保护作用。

BDE-209暴露引起男性生殖毒性，精子质量下降。然而，自噬在其中的作用尚不清楚。目的探讨海藻糖（Tre，自噬诱导剂）对BDE-209诱导的生殖损伤的保护作用及其可能机制。用2% w/v tre3和75 mg/kg/d BDE-209共处理小鼠42天。GC-2 spd细胞用Tre、氯喹（CQ，抑制自噬通量）、化合物C （CC， AMPK抑制剂）和BDE-209共处理。在bde -209暴露小鼠的性器官比例下降和精子质量差的情况下，tre3摄入量显著恢复。通过改善bde -209暴露小鼠异常的组蛋白-鱼精蛋白交换，补充Tre挽救了精子头部畸形。然而，tre3的摄入并不能恢复顶体的生物发生。此外，补充tre3可改善BDE-209致睾丸损伤。BDE-209阻断自噬通量，增加P62和LC3BⅡ/Ⅰ水平。机制上，CQ处理加重了BDE-209诱导的P62和LC3BⅡ/Ⅰ水平升高，而CC和Tre处理则抑制了BDE-209诱导的p-AMPK、p-ULK1、P62和LC3BⅡ/Ⅰ水平升高。补充tre3可通过AMPK-ULK1信号通路调节bde -209暴露小鼠的自噬流量，改善生殖损伤，为雄性生殖毒性提供新的理论基础和可能的治疗靶点。

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来源期刊

Food and Chemical Toxicology 工程技术-毒理学

CiteScore

10.90

自引率

4.70%

发文量

651

审稿时长

31 days

期刊介绍： Food and Chemical Toxicology (FCT), an internationally renowned journal, that publishes original research articles and reviews on toxic effects, in animals and humans, of natural or synthetic chemicals occurring in the human environment with particular emphasis on food, drugs, and chemicals, including agricultural and industrial safety, and consumer product safety. Areas such as safety evaluation of novel foods and ingredients, biotechnologically-derived products, and nanomaterials are included in the scope of the journal. FCT also encourages submission of papers on inter-relationships between nutrition and toxicology and on in vitro techniques, particularly those fostering the 3 Rs. The principal aim of the journal is to publish high impact, scholarly work and to serve as a multidisciplinary forum for research in toxicology. Papers submitted will be judged on the basis of scientific originality and contribution to the field, quality and subject matter. Studies should address at least one of the following: -Adverse physiological/biochemical, or pathological changes induced by specific defined substances -New techniques for assessing potential toxicity, including molecular biology -Mechanisms underlying toxic phenomena -Toxicological examinations of specific chemicals or consumer products, both those showing adverse effects and those demonstrating safety, that meet current standards of scientific acceptability. Authors must clearly and briefly identify what novel toxic effect (s) or toxic mechanism (s) of the chemical are being reported and what their significance is in the abstract. Furthermore, sufficient doses should be included in order to provide information on NOAEL/LOAEL values.