Improvement of Dentin Growth Parameters (Beta-catenin, bFGF, CD105, and BMP4) with Propolis as Adjuvant in Dental Caries Treatment.

Q1 Dentistry European Journal of Dentistry Pub Date : 2024-12-10 DOI:10.1055/s-0044-1791939

Retno Pudji Rahayu, Nirawati Pribadi, Ira Widjiastuti, Nur Ariska Nugrahani

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Abstract

Objective: The purpose of the study was to evaluate the efficacy of calcium hydroxide (Ca(OH)₂) and propolis in pulp capping for dental caries treatment, focusing on dentin growth parameters. The study also aims to determine the role of propolis as a natural adjuvant therapy in enhancing reparative dentin development while emphasizing the importance of proper technique and material preparation with markers for the expression of beta-catenin, bFGF, CD105, and BMP4.

Materials and methods: The left bottom molar teeth from 28 Wistar rats were divided into four groups. The first group, the control group, was given only aqua dest, and the second group received drilling treatment and additional therapies with Ca(OH)₂ (Ca(OH)₂) 0.625 μg. The third group was given drilling treatment and additional therapies with a combination of propolis with Ca(OH)₂ 0.781 μg until day 7. Finally, the fourth group received a combination of propolis with Ca(OH)₂ 0.781 μg until day 14. This research analyzed the expression of essential basic fibroblast growth factor (bFGF), CD105, beta-catenin, and bone morphogenetic protein 4 (BMP4).

Results: This research reports that the average expression of BMP4 and bFGF showed a significant result in treatment with additional therapies with propolis and Ca(OH)₂. The experiment indicates that propolis and Ca(OH)₂ could induce reparative dentine on days 7 and 14.

Conclusion: Propolis as an adjuvant shows better reparative dental formation with improvement in the expression of bFGF and BMP4 in 14 days of therapy.

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蜂胶辅助治疗龋病对牙本质生长参数（β -catenin、bFGF、CD105和BMP4）的改善

目的：评价氢氧化钙（Ca(OH)2）和蜂胶盖髓治疗龋病的效果，重点观察牙本质生长参数。本研究还旨在确定蜂胶作为天然辅助疗法在促进修复性牙本质发育中的作用，同时强调适当的技术和材料制备的重要性，标记β -catenin、bFGF、CD105和BMP4的表达。材料与方法：28只Wistar大鼠左下磨牙分为4组。第一组为对照组，仅给予水治疗，第二组在钻孔治疗的基础上加用钙（OH）2 (Ca(OH)2) 0.625 μg。第三组小鼠给予钻孔治疗和蜂胶与Ca(OH)2 0.781 μg联合治疗，至第7天。最后，第4组以Ca(OH)2 0.781 μg的蜂胶配伍至第14天。本研究分析了必需碱性成纤维细胞生长因子（bFGF）、CD105、β -连环蛋白（β -catenin）和骨形态发生蛋白4 （BMP4）的表达。结果：本研究报道，在蜂胶和Ca(OH)2的辅助治疗中，BMP4和bFGF的平均表达有显著的结果。实验结果表明，蜂胶和Ca(OH)2能在第7天和第14天诱导牙本质修复。结论：蜂胶作为辅助剂治疗14 d后，bFGF和BMP4的表达均有改善，具有较好的修复性牙形成效果。

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来源期刊

European Journal of Dentistry Dentistry-Dentistry (all)

CiteScore

5.10

自引率

0.00%

发文量

161

期刊介绍： The European Journal of Dentistry is the official journal of the Dental Investigations Society, based in Turkey. It is a double-blinded peer-reviewed, Open Access, multi-disciplinary international journal addressing various aspects of dentistry. The journal''s board consists of eminent investigators in dentistry from across the globe and presents an ideal international composition. The journal encourages its authors to submit original investigations, reviews, and reports addressing various divisions of dentistry including oral pathology, prosthodontics, endodontics, orthodontics etc. It is available both online and in print.