Thermal modulation of insecticide-induced hormetic and oxidative responses in insect pests.

Abstract

Environmental global changes are dramatically affecting agroecosystems. Insects have been shown to present various responses to multi-stress conditions (i.e., increase in temperature and exposure to contaminants). However, there is a knowledge gap on how temperature can modulate the hormetic effects in individuals sublethally exposed to chemical stressors. Here, we investigated how temperature (15, 20, 25, and 28 °C) modulates the effects of lethal and sublethal exposure to insecticides (imidacloprid) on the longevity, fecundity, and oxidative stress of a pest insect, the aphid Mysus persicae. Our results showed additive and interactive effects of temperature and insecticide on the stimulatory and oxidative responses of the insect pest. Overall, imidacloprid was 2.4-fold less toxic at 15 °C (3.547 μg/ml) than at 20 °C (1.482 μg/ml) and 24.6 to 19.8-fold less toxic than at 25 °C (0.144 μg/ml) and 28 °C (0.179 μg/ml) respectively. Furthermore, although the exposure of female aphids to most sublethal concentrations resulted in a decrease in their longevity and fecundity compared to the control, some of the sublethal concentrations produced positive effects in these parameters for the exposed individuals. The magnitude of induced sublethal effects varied between temperatures and occurred in similar ranges of low concentrations at temperatures 15 °C and 20 °C, and at temperatures 25 °C and 28 °C. Additionally, imidacloprid low concentrations induced a temperature-dependent production of reactive oxygen species in exposed insects at 12 and 24 h after exposure indicating oxidative stress. Our study supplies valuable data on how temperature modulates pesticide-mediated hormesis that can alter ecological interactions and functions within agroecosystems with potential implications in pest management.